ROC2(RNF7) (NM_014245) Human Over-expression Lysate
CAT#: LC402297
RNF7 HEK293T cell transient overexpression lysate (as WB positive control)
Product Datasheet for 'LC402297'
View other "RNF7" proteins (3)
Product images

Specifications
Product Data | |
Description | RNF7 HEK293T cell transient overexpression lysate (as WB positive control) |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC203831 |
Tag | C-MYC/DDK |
Detection Antibodies | Anti-DDK Antibody (TA50011-100) |
Accession Number | NM_014245, NP_055060 |
Other Names | RNF7, CKBBP1; ROC2; SAG |
Components | 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reference Data | |
RefSeq | NP_055060 |
Locus ID | 9616 |
Cytogenetics | CKBBP1; ROC2; SAG |
Protein Families | Druggable Genome |
Protein Pathways | Ubiquitin mediated proteolysis |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
PH303831 | RNF7 MS Standard C13 and N15-labeled recombinant protein (NP_055060) |
USD 1,995.00 |
|
LY402297 | Transient overexpression lysate of ring finger protein 7 (RNF7), transcript variant 1 |
USD 315.00 |
|
TP303831 | Recombinant protein of human ring finger protein 7 (RNF7), transcript variant 1 |
USD 748.00 |