SERPINB2 (NM_002575) Human Over-expression Lysate

CAT#: LC400915

SERPINB2 HEK293T cell transient overexpression lysate (as WB positive control)


 Product Datasheet for 'LC400915'

  View other "SERPINB2" proteins (10)

USD 95.00


Availability*
In Stock

Size
    • 20 ug


Product images

Specifications

Product Data
Description SERPINB2 HEK293T cell transient overexpression lysate (as WB positive control)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203139
Tag C-MYC/DDK
Detection Antibodies Anti-DDK Antibody (TA50011-100)
Accession Number NM_002575, NP_002566
Other Names SERPINB2, HsT1201; PAI; PAI-2; PAI2; PLANH2
Predicted MW 46.6 kDa
Components 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
RefSeq NP_002566
Locus ID 5055
Cytogenetics HsT1201; PAI; PAI-2; PAI2; PLANH2
Protein Families Secreted Protein, Druggable Genome

Other Versions

Other products for "SERPINB2"
Frequently bought together (3)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

USD 299.00


SERPINB2 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)
    • 100 ul

USD 379.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 150.00

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
40% off proteins and antibodies
68 Mouse Clones