Clathrin light chain (CLTB) (NM_007097) Human Mass Spec Standard

CAT#: PH324622

CLTB MS Standard C13 and N15-labeled recombinant protein (NP_009028)

Product Images

Coomassie blue staining of purified CLTB protein (Cat# TP324622). The protein was produced from HEK293T cells transfected with CLTB cDNA clone (Cat# RC224622) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC224622
• Predicted MW: 25 kDa
• Protein Sequence:
  Sequence Link

  >RC224622 representing NM_007097
  Red=Cloning site Green=Tags(s)
  
  MADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGT
  TVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWN
  QRQSEQVEKNKINNRIADKAFYQQPDADIIGYVASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCK
  DVSRLRSVLMSLKQTPLSR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_009028
• RefSeq Size: 1184
• RefSeq ORF: 687
• Synonyms: LCB
• Locus ID: 1212
• UniProt ID: P09497
• Cytogenetics: 5q35.2
• Summary: Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
• Protein Pathways: Endocytosis, Huntington's disease, Lysosome