H2BC11 (NM_021058) Human Mass Spec Standard

CAT#: PH311898

HIST1H2BJ MS Standard C13 and N15-labeled recombinant protein (NP_066402)

Product Images

Coomassie blue staining of purified HIST1H2BJ protein (Cat# TP311898). The protein was produced from HEK293T cells transfected with HIST1H2BJ cDNA clone (Cat# RC211898) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211898
• Predicted MW: 13.7 kDa
• Protein Sequence:
  Sequence Link

  >RC211898 representing NM_021058
  Red=Cloning site Green=Tags(s)
  
  MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDI
  FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_066402
• RefSeq Size: 481
• RefSeq ORF: 378
• Synonyms: H2B/r; H2BFR; H2BJ; HIST1H2BJ
• Locus ID: 8970
• UniProt ID: P06899, A0A024RCJ2
• Cytogenetics: 6p22.1
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015]
• Protein Pathways: Systemic lupus erythematosus