Cofilin 1 (CFL1) (NM_005507) Human Mass Spec Standard

CAT#: PH303585

CFL1 MS Standard C13 and N15-labeled recombinant protein (NP_005498)

Product Images

Coomassie blue staining of purified CFL1 protein (Cat# TP303585). The protein was produced from HEK293T cells transfected with CFL1 cDNA clone (Cat# RC203585) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203585
• Predicted MW: 18.5 kDa
• Protein Sequence:
  Sequence Link

  >RC203585 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYAT
  FVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCY
  EEVKDRCTLAEKLGGSAVISLEGKPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_005498
• RefSeq Size: 1260
• RefSeq ORF: 498
• Synonyms: CFL; cofilin; HEL-S-15
• Locus ID: 1072
• UniProt ID: P23528, V9HWI5
• Cytogenetics: 11q13.1
• Summary: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton