PAAF1 (NM_025155) Human Mass Spec Standard

CAT#: PH302994

PAAF1 MS Standard C13 and N15-labeled recombinant protein (NP_079431)

Product Images

Coomassie blue staining of purified PAAF1 protein (Cat# TP302994). The protein was produced from HEK293T cells transfected with PAAF1 cDNA clone (Cat# RC202994) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC202994
• Predicted MW: 42.2 kDa
• Protein Sequence:
  Sequence Link

  >RC202994 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPLRIQSDWAQALRKDEGEAWLSCHPPGKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHIS
  CPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCC
  RFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSGC
  LGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGS
  DAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQD
  LDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_079431
• RefSeq Size: 1626
• RefSeq ORF: 1176
• Synonyms: PAAF; Rpn14; WDR71
• Locus ID: 80227
• UniProt ID: Q9BRP4
• Cytogenetics: 11q13.4
• Summary: This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]