SNRPF (NM_003095) Human Mass Spec Standard

CAT#: PH300416

SNRPF MS Standard C13 and N15-labeled recombinant protein (NP_003086)

Product Images

Coomassie blue staining of purified SNRPF protein (Cat# TP300416). The protein was produced from HEK293T cells transfected with SNRPF cDNA clone (Cat# RC200416) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200416
• Predicted MW: 9.7 kDa
• Protein Sequence:
  Sequence Link

  >RC200416 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVL
  YIRGVEEEEEDGEMRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_003086
• RefSeq Size: 806
• RefSeq ORF: 258
• Synonyms: Sm-F; SMF; snRNP-F
• Locus ID: 6636
• UniProt ID: P62306
• Cytogenetics: 12q23.1
• Summary: Plays role in pre-mRNA splicing as core component of the SMN-Sm complex that mediates spliceosomal snRNP assembly and as component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:18984161, PubMed:19325628, PubMed:23333303, PubMed:25555158, PubMed:26912367, PubMed:28502770, PubMed:28781166, PubMed:28076346). Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes (PubMed:11991638, PubMed:28502770, PubMed:28781166, PubMed:28076346). Is also a component of the minor U12 spliceosome (PubMed:15146077). As part of the U7 snRNP it is involved in histone 3'-end processing (PubMed:12975319).[UniProtKB/Swiss-Prot Function]
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome