YKT6 (NM_006555) Human Mass Spec Standard

CAT#: PH300260

YKT6 MS Standard C13 and N15-labeled recombinant protein (NP_006546)

Product Images

Coomassie blue staining of purified YKT6 protein (Cat# TP300260). The protein was produced from HEK293T cells transfected with YKT6 cDNA clone (Cat# RC200260) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200260
• Predicted MW: 22.4 kDa
• Protein Sequence:
  Sequence Link

  >RC200260 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLYSLSVLYKGEAKVVLLKAAYDVSSFSFFQRSSVQEFMTFTSQLIVERSSKGTRASVKEQDYLCHVYV
  RNDSLAGVVIADNEYPSRVAFTLLEKVLDEFSKQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMT
  KVQAELDETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006546
• RefSeq Size: 2783
• RefSeq ORF: 594
• Locus ID: 10652
• UniProt ID: O15498, A4D2J0
• Cytogenetics: 7p13
• Summary: This gene product is one of the SNARE recognition molecules implicated in vesicular transport between secretory compartments. It is a membrane associated, isoprenylated protein that functions at the endoplasmic reticulum-Golgi transport step. This protein is highly conserved from yeast to human and can functionally complement the loss of the yeast homolog in the yeast secretory pathway. [provided by RefSeq, Jul 2008]
• Protein Pathways: SNARE interactions in vesicular transport