14-3-3 epsilon (YWHAE) (NM_006761) Human Mass Spec Standard

CAT#: PH300245

YWHAE MS Standard C13 and N15-labeled recombinant protein (NP_006752)

Product Images

Coomassie blue staining of purified YWHAE protein (Cat# TP300245). The protein was produced from HEK293T cells transfected with YWHAE cDNA clone (Cat# RC200245) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200245
• Predicted MW: 29.2 kDa
• Protein Sequence:
  Sequence Link

  >RC200245 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKE
  ENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGND
  RKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLS
  EESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006752
• RefSeq Size: 1827
• RefSeq ORF: 765
• Synonyms: 14-3-3E; HEL2; KCIP-1; MDCR; MDS
• Locus ID: 7531
• UniProt ID: P62258, V9HW98
• Cytogenetics: 17p13.3
• Summary: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis