Nucleolin(NCL) Human Knockout Lysate (Locus ID 4691)

CAT#: LC810150

NCL-knockout Cell lysate from HeLa cell ine, one vial of parental cell line lysate included as control


 Product Datasheet for 'LC810150'

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USD 495.00


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Size
    • 100 ug


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Specifications

Product Data
Description NCL-knockout Cell lysate from HeLa cell ine, one vial of parental cell line lysate included as control
Species Human
Parental Cell Line HeLa
Symbol NCL
Other Names NCL, C23; C23; Nsr1
Components 1 vial of 100 ug KO cell lysate (lyophilized)
1 vial of 100ug parental cell lysate (lyophilized)
Storage The lysate is shipped at room temperature. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Preparation Parental cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The KO cell lysates are the cell homogenate in RIPA buffer made from the KO cell lines. The protein concentration was determined with BCA assay. A vial of lysate from the parental cell line was also included as an internal control. To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
Locus ID 4691
Protein Families Stem cell - Pluripotency, Druggable Genome
Protein Pathways Pathogenic Escherichia coli infection

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