SK-MEL-2 (Human Skin Melanoma) Whole Cell Lysate

CAT#: LY500049

Specifications

Product Data

• Tissue: Skin
• Host: Human
• Lysis Buffer: Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4)
• Component: 1 vial of 100ug whole cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -80ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Recommended Application: WB, IP

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.