HT29 (Human Colon Colorectal Adenocarcinoma) Whole Cell Lysate
HT29 (Human Colon Colorectal Adenocarcinoma) Whole Cell LysateRequest Larger Size Package
|Lysis Buffer||Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4)|
|Component||1 vial of 100ug whole cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
|Storage||The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -80ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
|Recommended Application||WB, IP|
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