NCI-H460 (Human Large Cell Lung Carcinoma) Whole Cell Lysate
NCI-H460 (Human Large Cell Lung Carcinoma) Whole Cell LysateBulk Request/Custom Protein Development
|Lysis Buffer||Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4)|
|Component||1 vial of 100ug whole cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
|Storage||The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -80ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
|Recommended Application||WB, IP|
|The use of this Proteins has been cited in the following citations:|
Matrix Metalloproteinase-19 Promotes Metastatic Behavior In Vitro and is Associated with Increased Mortality in Non-small Cell Lung Cancer
,Yu, G;Herazo-Maya, JD;Nukui, T;Romkes, M;Parwani, A;Juan-Guardela, BM;Robertson, J;Gauldie, J;Siegfried, JM;Kaminski, N;Kass, DJ;,
Am. J. Respir. Crit. Care Med. Sep 2014
,PubMed ID 25250855