Stable Cell Line

CAT#: SL500060

Human Ghrelin Receptor GHSR Stable cell Line - CHO-K1

Product images

Figure 1. Calcium dose-response for ghrelin stimulation of CHO/GHSR cells compared to untransfected CHO-K1 cells. The passage 1and passage 3 of CHO/GHSR cells that stably express the GHSR (Green and blue circles) or untransfected CHO-K1 cells which is the same passage as CHO/GHSR MCB (black triangle) were loaded with Fluo-4 NW (Invitrogen), then stimulated with the indicated concentration of ghrelin. The change in intracellular calcium was measured by a FlexStation III fluorescent plate reader. Data poins represent the average+/- standard deviation of triplicate determinations.

Specifications

Product Data

• Gene: GHSR
• Accn: BC069068
• Synonyms: GHDP
• Host Cell: CHO-K1
• Format: Cryopreserved cells, 6 x 10^6 cells/vial
• Mycoplasma: Negative by MycoAlert Kit, Lonza
• Cell Line Validation: Function assay
• Background: The growth hormone secretagagogue receptor (GHSR), also known as GHSR subtype 1a (GHSR1a), is a Gq/11 protein-coupled receptor that is widely expressed in peripheral tissues and various regions of the brain such as the pituitary gland, hypothalamus, thalamus, cortex, and hippocampus. GHSR plays a critical role in maintaining energy balance and neuronal health and is a characterized therapeutic target for obesity and metabolic syndrome.