Papss2 Mouse Gene Knockout Kit (CRISPR)
Papss2 - mouse gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN312800G1, Papss2 gRNA vector 1 in pCas-Guide vector
KN312800G2, Papss2 gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||BC090997, NM_001201470, NM_011864, XM_006527064|
|Summary||Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth. [UniProtKB/Swiss-Prot Function]|
|Gene Expression Resources|