Nt5e Mouse Gene Knockout Kit (CRISPR)

CAT#: KN311280

Nt5e - mouse gene knockout kit via CRISPR

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 HDR-mediated knockout kit validation

USD 1,290.00


Availability*
4 Weeks

Size
    • 1 kit

Product images

Specifications

Product Data
Symbol Nt5e
Locus ID 23959
Kit Components

KN311280G1, Nt5e gRNA vector 1 in pCas-Guide vector

KN311280G2, Nt5e gRNA vector 2 in pCas-Guide vector

KN311280-D, donor DNA containing Left and right homologous arms and GFP-puro functional cassette.

GE100003, scramble sequence in pCas-Guide vector

Disclaimer The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq BC119268, BC119270, NM_011851
Synonyms 2210401F01Rik; 5'-NT; AI447961; CD73; eNT; NT; Nt5
Summary This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016].
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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