Nt5e Mouse Gene Knockout Kit (CRISPR)
Nt5e - mouse gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN311280G1, Nt5e gRNA vector 1 in pCas-Guide vector
KN311280G2, Nt5e gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||BC119268, BC119270, NM_011851|
|Synonyms||2210401F01Rik; 5'-NT; AI447961; CD73; eNT; NT; Nt5|
|Summary||This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016].|
|Gene Expression Resources|