APAF1 Human Gene Knockout Kit (CRISPR)
APAF1 - human gene knockout kit via CRISPRchange donor?
KN212252G1, APAF1 gRNA vector 1 in pCas-Guide vector,
KN212252G2, APAF1 gRNA vector 2 in pCas-Guide vector,
donor vector containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||NM_001160, NM_013229, NM_181861, NM_181868, NM_181869, XM_017019250, XR_429095|
|Summary||This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]|
|Gene Expression Resources|
"APAF1" in other Product Lines
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