PLCH1 Human Gene Knockout Kit (CRISPR)
PLCH1 - human gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN210686G1, PLCH1 gRNA vector 1 in pCas-Guide vector
KN210686G2, PLCH1 gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||NM_001130960, NM_001130961, NM_001349250, NM_001349251, NM_001349252, NM_014996, XM_005247238, XM_005247239, XM_011512560, XM_011512561, XM_011512562, XM_011512564, XM_011512565, XM_011512566, XM_011512567, XM_017005923, XM_017005924, XM_017005925, XM_017005926, XM_017005927, XM_017005928, XM_017005929|
|Summary||PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009].|
|Gene Expression Resources|