AKAP8 Human Gene Knockout Kit (CRISPR)
AKAP8 - human gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN205349G1, AKAP8 gRNA vector 1 in pCas-Guide vector
KN205349G2, AKAP8 gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||NM_005858, XM_011527624, XM_017026141, XR_001753582|
|Synonyms||AKAP 95; AKAP-8; AKAP-95; AKAP95|
|Summary||This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011].|
|Gene Expression Resources|