HNF1 beta (HNF1B) Human Gene Knockout Kit (CRISPR)

CAT#: KN204453

HNF1B - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: HNF1 beta
• Locus ID: 6928
• Components:

  KN204453G1, HNF1 beta gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GAGCGCCCTGCTGAGCTCCG

  KN204453G2, HNF1 beta gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CAGCACCTCCTTGGTGACCC

  KN204453D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AGGGTCTCTA CTCTTCGCTT CCTCCTGCGT CCCCTTCTTC CACGAGCACC CCAGCTTCCT CTGGCTCTCA CCTGAACCCC GAAGCGTAGT GTCTTCTCTC TGGACTAAAG CGGAACTGAG AACCGGTGGA AAAGCCCCGC GCCTAGGCTG CAAGGCACTG GCTTAACAAG TCCAAAGGTT AGGTGAAGTT TGGCTGATAA GCAGAACCAG TAAAAGAAGG TCTCTAGCCC CCCAGCGTGA GTACAATGGA CCCTGGCAAA GCCCCGCTCC CGGCCCAGGT CTTCTGCTCT CCAGGTCTGC CCCTCCGGCT CTCCCTCTCT CCGGGTTTCC CCCTCCCCAC CATCATTTGC ATCCAGCCGA AAGCTGGGCC CTTCCCACTA ATTTGCATAT CTTATATGGC CTAATGGTGG CGATCATGGC AAGTTAGAAG TTTTCTGACT CCTTTCGGAG GAGCCTCCGG GACCCCGGGG AGTAACAGGT GTCTGGAGGC TGAAGGGTGG AGGGGTTCCT GGATTTGGGG TTTGCTTGTG AAACTCCCCT CCACCCTCCT CTCTCGCACC CACCCACCCC CTCACCCCCT TCTTTTTCCG TCCTTGGAAA GAGTTGCTGC CATCCCCGAA CTTCGGGGTG AAGCTGGAGA CGCTGCCCCT GTCCCCTGGC AGCGGGGCCG AGCCCGACAC CAAGCCGGTC TTCCATACTC TCACCAACGG CCACGCCAAG GGCCGCTTGT CCGGCGACGA GGGCTCCGAG GACGGCGACG ACTATGACAC ACCTCCCATC CTCAAGGAGC TGCAGGCGCT CAACACCGAG GAGGCGGCGG AGCAGCGGGC GGAGGTGGAC CGGATGCTCA GGTAGGCGCA GAGCGAGGTG GAGGGGACCC ACCCGAACCC CTGGAGCCCC GGCCCCGGGC CTGAGTGACA CTGCGCCCGA CCACACTCGC CAAGCCCGTT TCCCACCAGA GAAGTCCCCC GGGGGGCGCT CTGCTTCTCT CCCAACACCC GGACCCTTCC CAATCCCTTA GCGGGACGAC CCTGCGGCCC ACCGGGCTTC TTCTCCCCAG GCCCAGGCCA GCGTCCGAGA CCCAAGGGCT TTTCCCCGCG GGGCTGGGGC TGGGCCGGGG AGAACTGGAG CTGTGGAGCC CTTTGCCGGG AGGTTTGCCC ACGCGACTGA AGGTGCGAGT GGGTGCAGTG AAACCGCTGC

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000458, NM_001165923, NM_001304286, NM_006481
• UniProt ID: P35680
• Synonyms: FJHN; HNF-1-beta; HNF-1B; HNF1beta; HNF2; HPC11; LF-B3; LFB3; MODY5; TCF-2; TCF2; VHNF1
• Summary: This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]