CA12 Human Gene Knockout Kit (CRISPR)
CA12 - human gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN204034G1, CA12 gRNA vector 1 in pCas-Guide vector
KN204034G2, CA12 gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||NM_001218, NM_001293642, NM_206925, NR_135511|
|Synonyms||CA-XII; CAXII; HsT18816; T18816|
|Summary||Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014].|
|The use of this Gene Expression has been cited in the following citations:|
P-glycoprotein-mediated chemoresistance is reversed by carbonic anhydrase XII inhibitors
,Kopecka, J;Rankin, GM;Salaroglio, IC;Poulsen, SA;Riganti, C;,
,PubMed ID 27811376
|Gene Expression Resources|