MRPL43 Human Gene Knockout Kit (CRISPR)
MRPL43 - human gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN203936G1, MRPL43 gRNA vector 1 in pCas-Guide vector
KN203936G2, MRPL43 gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||AK095556, NM_001308396, NM_032112, NM_176792, NM_176793, NM_176794, XM_005270231, XM_006718035, XM_017016790, XM_046760, XR_246111|
|Synonyms||bMRP36a; L43mt; MRP-L43|
|Summary||Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for a semaphorin class 4 protein (SEMA4G) overlap at map location 10q24.31 and are transcribed in opposite directions. Sequence analysis identified multiple transcript variants encoding at least four different protein isoforms. [provided by RefSeq, Jul 2008].|
|Gene Expression Resources|