c20orf72 (MGME1) Human Gene Knockout Kit (CRISPR)

CAT#: KN203886

MGME1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: c20orf72
• Locus ID: 92667
• Components:

  KN203886G1, c20orf72 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: AGACCATTTGCAGGCAGCTC

  KN203886G2, c20orf72 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGTGGAATCAGCTGCCCTTG

  KN203886D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  CGGGAGGCGA CTTTTTGCGG TCACAGTAAT GGGACCTCAG CGCCGCCACC AGCTCTGACC GGCGAGCGAC GGGACCCTCC AACCCGATTT CCACGTCTGT GCAACGGGTT CATTCTAGAC CCACGGTAGA GGGTGTGAGG ACCAAATGAG AAGTATCCAC ACAGAATACT TAGCAGAGAG CCTGGTGTCT AGGAATTAGT CGTCGTCTGT CACCTAGGAC TGTGTGGCCG CCATGGGACG GGCTCCATCT TTGTGTTTTT CCGTGTCTCG CACACGCCTC CCTTCCTGGG CGTCACATTC CTGCAGATTA GAAACTGGGA CGTCTTGCCT CAAGCTGTCT ACTAATTCGC ATTTAGTATG TGTCGGTCCT TCGGGTATTG AGTCTTGATT TATTTATAAA TTTTTTTTGG AGGCAGGGTC TCGCTCTGTC GACTTTCTTA AGAAAGGGCT CTGTCCAAAC ATGTCGAAAA AGTTCACAAT TTTGATGTGC AATATCATTC TGATGCAGCT TTCGCTTTGA TGGTTGTTTT CTCTCCAATA GGAATACAAA CATAAAGGCC TTCGACCGTT GCAAATAGAC TAAAGTGAAA ACAAATCTGA TGGCTTTCTC TACTTCCTCT TACTCATGTG GCCGGAAGAA AAAAGTGAAC CCATATGAAG AAGTGGACCA AGAAAAATAC TCTAATTTAG TTCAGTCTGT CTTGTCATCC AGAGGCGTCG CCCAGACCCC GGGATCGGTG GAGGAAGATG CTTTGCTCTG TGGACCCGTG AGCAAGCATA AGCTGCCAAA CCAAGGTGAG GACAGACGAG TGCCACAAAA CTGGTTTCCT ATCTTCAATC CAGAGAGAAG TGATAAACCA AATGCAAGTG ATCCTTCAGT TCCTTTGAAA ATCCCCTTGC AAAGGAATGT GATACCAAGT GTGACCCGAG TCCTTCAGCA GACCATGACA AAACAACAGG TTTTCTTGTT GGAGAGGTGG AAACAGCGGA TGATTCTGGA ACTGGGAGAA GATGGCTTTA AAGAATACAC TTCAAGTAAT TATCTCAATT CTGATTCTAT ATGTTTGCTA TGTTTTCTAT AATAGAGAGC AACGGTGTCA AAAGAATGAG GTTTGGTTTT GTTTTTTTAA CTTACTAGCA AGGAACTCCC TTCAGATAGT AATTAACATT AAATAGCACT TTATGTGTAT TAACTCACTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001310338, NM_001310339, NM_052865, NM_001363738
• UniProt ID: Q9BQP7
• Synonyms: bA504H3.4; C20orf72; DDK1; MTDPS11
• Summary: The protein encoded by this gene is a nuclear-encoded mitochondrial protein necessary for the maintenance of mitochondrial genome synthesis. The encoded protein is a RecB-type exonuclease and primarily cleaves single-stranded DNA. Defects in this gene have been associated with mitochondrial DNA depletion syndrome-11. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]