IVD Human Gene Knockout Kit (CRISPR)
IVD - human gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN201077G1, IVD gRNA vector 1 in pCas-Guide vector
KN201077G2, IVD gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||NM_001159508, NM_001354597, NM_001354598, NM_001354599, NM_001354600, NM_001354601, NM_002225, NR_148923, NR_148924, NR_148925, XM_005254350, XM_006720492, XM_006720495, XM_017022149, XM_017022150, XM_017022151, XM_017022152, XM_017022153, XM_017022154, XM_017022155, XM_017022156, XM_017022157, XM_017022158, XR_001751260, XR_001751262, XR_001751263, XR_001751264, XR_429453|
|Summary||Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009].|
|Gene Expression Resources|