NCF2 Human Gene Knockout Kit (CRISPR)
NCF2 - human gene knockout kit via CRISPRchange donor?
HDR-mediated knockout kit validation
KN200704G1, NCF2 gRNA vector 1 in pCas-Guide vector
KN200704G2, NCF2 gRNA vector 2 in pCas-Guide vector
donor DNA containing Left and right homologous arms and GFP-puro functional cassette.
GE100003, scramble sequence in pCas-Guide vector
|Disclaimer||The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.|
|RefSeq||NM_000433, NM_001127651, NM_001190789, NM_001190794, XM_005245207, XM_011509580, XM_011509581|
|Synonyms||NCF-2; NOXA2; P67-PHOX; P67PHOX|
|Summary||This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010].|
|Gene Expression Resources|