Mouse Nemf activation kit by CRISPRa

CAT#: GA206659

Nemf CRISPRa kit - CRISPR gene activation of mouse nuclear export mediator factor

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Nemf
• Locus ID: 66244
• Kit Components:

  GA206659G1, Nemf gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA206659G2, Nemf gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA206659G3, Nemf gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_025441, NM_181665
• UniProt ID: Q8CCP0
• Synonyms: 1500011I12Rik; 4933405E14Rik; Sdccag1
• Summary: Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. Nemf is important for the stable association of Ltn1 to the complex. May indirectly play a role in nuclear export.[UniProtKB/Swiss-Prot Function]