PI 3 Kinase catalytic subunit alpha(PIK3CA) (NM_006218) Human Mutant ORF Clone

CAT#: RC400348

PIK3CA Mutant (E545K), Myc-DDK-tagged ORF clone of Homo sapiens phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA) as transfection-ready DNA

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  • TrueORF®

USD 1,020.00


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Size
    • 10 ug

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Specifications

Product Data
Product Name PI 3 Kinase catalytic subunit alpha(PIK3CA) (NM_006218) Human Mutant ORF Clone
Mutation Description E545K
Affected Codon# 545
Affected NT# c.1633
Nucleotide Mutation PIK3CA Mutant (E545K), Myc-DDK-tagged ORF clone of Homo sapiens phosphoinositide-3-kinase, catalytic, alpha polypeptide (PIK3CA) as transfection-ready DNA
Effect Missense
Symbol PIK3CA
Synonyms CLOVE; CWS5; MCAP; MCM; MCMTC; p110-alpha; PI3K; PI3K-alpha
Vector pCMV6-Entry
Tag Myc-DDK
ACCN NM_006218
ORF Size 3207 bp
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NP_006209
RefSeq Size 3724
RefSeq ORF 3207
Locus ID 5290
Cytogenetics 3q26.32
Domains PI3K_rbd, PI3_PI4_kinase, PI3Ka, PI3K_C2, PI3K_p85B
Protein Families Druggable Genome
Protein Pathways Inositol phosphate metabolism, ErbB signaling pathway, Chemokine signaling pathway, Phosphatidylinositol signaling system, mTOR signaling pathway, Apoptosis, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Leukocyte transendothelial migration, Neurotrophin signaling pathway, Regulation of actin cytoskeleton, Insulin signaling pathway, Progesterone-mediated oocyte maturation, Type II diabetes mellitus, Pathways in cancer, Colorectal cancer, Renal cell carcinoma, Pancreatic cancer, Endometrial cancer, Glioma, Prostate cancer, Melanoma, Chronic myeloid leukemia, Acute myeloid leukemia, Small cell lung cancer, Non-small cell lung cancer
MW 124 kDa
Gene Summary Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016].
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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