Axin 1 (AXIN1) (NM_003502) Human Mutant ORF Clone

CAT#: RC400175

AXIN1 Mutant (P345L), Myc-DDK-tagged ORF clone of Homo sapiens axin 1 (AXIN1), transcript variant 1 as transfection-ready DNA

Specifications

Product Data

• Mutation Description: P345L
• Affected Codon#: 345
• Affected NT#: c.1034
• Tag: Myc-DDK
• Effect: Missense
• Symbol: AXIN1
• Synonyms: AXIN; PPP1R49
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Vector: pCMV6-Entry
• ACCN: NM_003502
• ORF Size: 2586 bp
• Sequence Data:
  ORF Nucleotide Sequence

  >RC400175 representing NM_003502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATCCAAGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAGATGCTCCCCGACCCC
  CAGTGCCTGGTGAGGAGGGAGAACTGGTGTCCACAGACCCGAGGCCCGCCAGCTACAGTTTCTGCTCCGG
  GAAAGGTGTTGGCATTAAAGGTGAGACTTCGACGGCCACTCCGAGGCGCTCGGATCTGGACCTGGGGTAT
  GAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAAGTGGGCTGAGTCACTGCATTCCCTGCTGG
  ATGACCAAGATGGGATAAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGCCGACTTGCTGGACTT
  CTGGTTTGCCTGCACTGGCTTCAGGAAGCTGGAGCCCTGTGACTCGAACGAGGAGAAGAGGCTGAAGCTG
  GCGAGAGCCATCTACCGAAAGTACATTCTTGATAACAATGGCATCGTGTCCCGGCAGACCAAGCCAGCCA
  CCAAGAGCTTCATAAAGGGCTGCATCATGAAGCAGCTGATCGATCCTGCCATGTTTGACCAGGCCCAGAC
  CGAAATCCAGGCCACTATGGAGGAAAACACCTATCCCTCCTTCCTTAAGTCTGATATTTATTTGGAATAT
  ACGAGGACAGGCTCGGAGAGCCCCAAAGTCTGTAGTGACCAGAGCTCTGGGTCAGGGACAGGGAAGGGCA
  TATCTGGATACCTGCCGACCTTAAATGAAGATGAGGAATGGAAGTGTGACCAGGACATGGATGAGGACGA
  TGGCAGAGACGCTGCTCCCCCCGGAAGACTCCCTCAGAAGCTGCTCCTGGAGACAGCTGCCCCGAGGGTC
  TCCTCCAGTAGACGGTACAGCGAAGGCAGAGAGTTCAGGTATGGATCCTGGCGGGAGCCAGTCAACCCCT
  ATTATGTCAATGCCGGCTATGCCCTGGCCCCAGCCACCAGTGCCAACGACAGCGAGCAGCAGAGCCTGTC
  CAGCGATGCAGACACCCTGTCCCTCACGGACAGCAGCGTGGATGGGATCCCCCTATACAGGATCCGTAAG
  CAGCACCGCAGGGAGATGCAGGAGAGCGTGCAGGTCAATGGGCGGGTGCCCCTACCTCACATTCCCCGCA
  CGTACCGGGTGCCGAAGGAGGTCCGCGTGGAGCCTCAGAAGTTCGCGGAGGAGCTCATCCACCGCCTGGA
  GGCTGTGCAGCGCACGCGGGAGGCCGAGGAGAAGCTGGAGGAGCGGCTGAAGCGCGTGCGCATGGAGGAG
  GAAGGTGAGGACGGCGATCCATCGTCAGGGCCCCCAGGGCCGTGTCACAAGCTGCCTCCCGCCCCCGCTT
  GGCACCACTTCCCGCCCCGCTGTGTGGACATGGGCTGTGCCGGGCTCCGGGATGCACACGAGGAGAACCC
  TGAGAGCATCCTGGACGAGCACGTACAGCGTGTGCTGAGGACACCTGGCCGCCAGTCGCCTGGGCCTGGC
  CATCGCTCCCCGGACAGTGGGCACGTGGCCAAGATGCCAGTGGCACTGGGGGGTGCCGCCTCGGGGCACG
  GGAAGCACGTACCCAAGTCAGGGGCGAAGCTGGACGCGGCCGGCCTGCACCACCACCGACACGTCCACCA
  CCACGTCCACCACAGCACAGCCCGGCCCAAGGAGCAGGTGGAGGCCGAGGCCACCCGCAGGGCCCAGAGC
  AGCTTCGCCTGGGGCCTGGAACCACACAGCCATGGGGCAAGGTCCCGAGGCTACTCAGAGAGTGTTGGCG
  CTGCCCCCAACGCCAGTGATGGCCTCGCCCACAGTGGGAAGGTGGGCGTGGCGTGCAAAAGAAATGCCAA
  GAAGGCTGAGTCGGGGAAGAGCGCCAGCACCGAGGTGCCAGGTGCCTCGGAGGATGCGGAGAAGAACCAG
  AAAATCATGCAGTGGATCATTGAGGGGGAAAAGGAGATCAGCAGGCACCGCAGGACCGGCCACGGGTCTT
  CGGGGACGAGGAAGCCACAGCCCCATGAGAACTCCAGACCCTTGTCCCTTGAGCACCCCTGGGCCGGCCC
  TCAGCTCCGGACCTCCGTGCAGCCCTCCCACCTCTTCATCCAAGACCCCACCATGCCACCCCACCCAGCT
  CCCAACCCCCTAACCCAGCTGGAGGAGGCGCGCCGACGTCTGGAGGAGGAAGAAAAGAGAGCCAGCCGAG
  CACCCTCCAAGCAGAGGTATGTGCAGGAGGTTATGCGGCGGGGACGCGCCTGCGTCAGGCCAGCGTGCGC
  GCCGGTGCTGCACGTGGTACCAGCCGTGTCGGACATGGAGCTCTCCGAGACAGAGACAAGATCGCAGAGG
  AAGGTGGGCGGCGGGAGTGCCCAGCCGTGTGACAGCATCGTTGTGGCGTACTACTTCTGCGGGGAACCCA
  TCCCCTACCGCACCCTGGTGAGGGGCCGCGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTGACCAAAAA
  GGGCAGCTACAGATACTACTTCAAGAAAGTGAGCGACGAGTTTGACTGTGGGGTGGTGTTTGAGGAGGTT
  CGAGAGGACGAGGCCGTCCTGCCCGTCTTTGAGGAGAAGATCATCGGCAAAGTGGAGAAGGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC400175 representing NM_003502
  Red=Cloning site Green=Tags(s)
  
  MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGY
  EPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKL
  ARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEY
  TRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRV
  SSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPLYRIRK
  QHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEE
  EGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPG
  HRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQS
  SFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQ
  KIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPA
  PNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQR
  KVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEV
  REDEAVLPVFEEKIIGKVEKVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).

Reference Data

• RefSeq: NP_003493
• RefSeq Size: 3675 bp
• RefSeq ORF: 2589 bp
• Locus ID: 8312
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Wnt signaling pathway
• MW: 95 kDa
• Gene Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]