LAPTM4B (NM_018407) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC207325L2V

Lenti ORF particles, LAPTM4B (mGFP-tagged) - Human lysosomal protein transmembrane 4 beta (LAPTM4B), 200ul, >10^7 TU/mL

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: LAPTM4B
• Synonyms: LAPTM4beta; LC27
• Mammalian Cell Selection: None
• Vector: pLenti-C-mGFP
• ACCN: NM_018407
• ORF Size: 951 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC207325).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018407.4, NP_060877.3
• RefSeq Size: 2242 bp
• RefSeq ORF: 681 bp
• Locus ID: 55353
• UniProt ID: Q86VI4
• Cytogenetics: 8q22.1
• Domains: Mtp
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• MW: 35 kDa
• Gene Summary: Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).[UniProtKB/Swiss-Prot Function]