TFEB (NM_007162) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC207153L4V

Lenti ORF particles, TFEB (mGFP-tagged) - Human transcription factor EB (TFEB), transcript variant 1, 200ul, >10^7 TU/mL

Product Images

RC207153L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC207153L4V particle to overexpress human TFEB-mGFP fusion protein.

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  RC207153L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC207153L4V particle to overexpress human TFEB-mGFP fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: TFEB
• Synonyms: ALPHATFEB; BHLHE35; TCFEB
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_007162
• ORF Size: 1428 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC207153).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007162.1
• RefSeq Size: 2364 bp
• RefSeq ORF: 1431 bp
• Locus ID: 7942
• UniProt ID: P19484
• Cytogenetics: 6p21.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 52.9 kDa
• Gene Summary: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 (PubMed:29146937).[UniProtKB/Swiss-Prot Function]