Melanoma gp100 (PMEL) (NM_006928) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC200663L4V

Lenti ORF particles, PMEL (mGFP-tagged) - Human premelanosome protein (PMEL), transcript variant 3, 200ul, >10^7 TU/mL

Product Images

RC200663L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC200663L4V particle to overexpress human PMEL-mGFP fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: mGFP
• Symbol: PMEL
• Synonyms: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-mGFP-P2A-Puro
• ACCN: NM_006928
• ORF Size: 1983 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC200663).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006928.3
• RefSeq Size: 2143 bp
• RefSeq ORF: 1986 bp
• Locus ID: 6490
• UniProt ID: P40967
• Cytogenetics: 12q13.2
• Protein Families: Secreted Protein, Transmembrane
• MW: 70.1 kDa
• Gene Summary: This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]