Shh (NM_009170) Mouse Tagged ORF Clone Lentiviral Particle

CAT#: MR227201L3V

Lenti ORF particles, Shh (Myc-DDK-tagged) - Mouse sonic hedgehog (Shh), 200ul, >10^7 TU/mL

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Shh
• Synonyms: 9530036O11Rik; Dsh; Hhg1; Hx; Hxl3; M100081
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• ACCN: NM_009170
• ORF Size: 1311 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(MR227201).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009170.3, NP_033196.1
• RefSeq Size: 2727 bp
• RefSeq ORF: 1314 bp
• Locus ID: 20423
• UniProt ID: Q62226
• Cytogenetics: 5 14.39 cM
• Gene Summary: Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (PubMed:8824192, PubMed:7891723). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (PubMed:8824192). Both activities occur in the reticulum endoplasmic (PubMed:21357747). Once cleaved, ShhC is degraded in the endoplasmic reticulum (PubMed:21357747).[UniProtKB/Swiss-Prot Function]