HA (NC_002207) Virus Tagged ORF Clone

CAT#: VC102489

Myc-DDK-tagged ORF clone of viral ORF for hemagglutinin [Influenza B virus], codon optimized for human cell expression, NP_056660

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102489 represents NCBI reference of NP_056660 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCAATAATAGTACTCCTTATGGTGGTAACCTCTAACGCGGATAGAATTTGTACCGGGATCACTT
  CTAGCAACTCACCCCATGTGGTAAAGACCGCAACACAAGGCGAAGTAAACGTCACAGGGGTGATCCCTCT
  CACCACAACACCCACTAAGAGTCATTTTGCTAATCTGAAAGGGACTCAGACGCGGGGGAAACTCTGTCCC
  AATTGCTTTAATTGTACAGATCTGGATGTAGCCTTGGGGAGGCCTAAATGTATGGGGAATACCCCTAGCG
  CAAAGGTATCTATACTGCACGAAGTAAAACCCGCAACTTCTGGGTGCTTTCCTATAATGCACGATCGCAC
  TAAGATCCGGCAACTTCCAAACCTCCTGCGGGGATACGAGAATATACGGCTCTCCACAAGCAACGTGATT
  AATACAGAAACTGCCCCTGGCGGCCCTTACAAAGTTGGAACCTCCGGGAGTTGTCCAAATGTGGCGAATG
  GAAATGGCTTCTTTAACACGATGGCGTGGGTCATTCCAAAGGACAATAACAAAACCGCTATTAATCCTGT
  GACAGTCGAGGTGCCATATATCTGCTCCGAAGGAGAGGATCAGATAACAGTCTGGGGTTTCCATAGCGAC
  GATAAGACGCAGATGGAAAGACTGTATGGGGATTCAAATCCCCAGAAGTTTACTTCTAGCGCTAATGGAG
  TTACTACCCATTATGTAAGCCAGATTGGGGGCTTTCCTAATCAAACCGAAGACGAAGGCCTCAAGCAGTC
  TGGAAGGATCGTCGTTGACTACATGGTGCAAAAGCCTGGCAAGACAGGCACCATAGTGTATCAGAGAGGA
  ATCCTCCTCCCCCAGAAGGTCTGGTGCGCTAGCGGAAGGAGTAAGGTTATTAAAGGGAGTCTCCCCCTGA
  TTGGCGAAGCTGATTGTCTGCATGAGAAATATGGGGGTTTGAATAAGAGTAAGCCATACTACACAGGCGA
  ACACGCCAAGGCGATCGGGAACTGCCCGATTTGGGTCAAGACTCCGCTGAAGCTGGCCAACGGTACGAAA
  TACAGGCCTCCAGCAAAACTGCTGAAAGAGAGGGGCTTCTTCGGGGCAATTGCCGGATTCCTGGAAGGCG
  GGTGGGAAGGTATGATTGCGGGCTGGCATGGCTACACATCTCATGGGGCGCACGGCGTAGCCGTGGCAGC
  TGACCTTAAGAGTACCCAGGAGGCGATCAACAAGATCACCAAGAACCTGAATTATCTGAGCGAGCTGGAA
  GTAAAAAACCTGCAGCGATTGAGCGGGGCTATGAATGAGCTCCACGACGAGATTTTGGAGCTTGACGAAA
  AGGTGGATGATCTTCGGGCGGACACCATCTCCTCCCAGATCGAACTCGCCGTACTGCTGTCTAACGAGGG
  AATTATTAACTCAGAGGACGAACACCTCCTGGCTTTGGAACGCAAACTGAAGAAAATGCTTGGTCCGTCT
  GCGGTGGAAATTGGTAATGGGTGCTTCGAGACCAAACATAAATGTAACCAAACCTGTCTGGATCGGATTG
  CCGCCGGTACCTTTAACGCAGGTGACTTCTCCCTTCCCACCTTCGACAGCCTGAACATCACAGCAGCCAG
  CCTGAATGACGATGGATTGGACAACCACACCATCTTGCTTTATTATTCCACCGCCGCTTCCTCCCTGGCC
  GTGACACTCATGATCGCCATCTTCATCGTCTATATGGTGTCAAGGGACAATGTCTCTTGTAGCATTTGCC
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102489 representing NP_056660
  Red=Cloning sites Green=Tags
  
  MKAIIVLLMVVTSNADRICTGITSSNSPHVVKTATQGEVNVTGVIPLTTTPTKSHFANLKGTQTRGKLCP
  NCFNCTDLDVALGRPKCMGNTPSAKVSILHEVKPATSGCFPIMHDRTKIRQLPNLLRGYENIRLSTSNVI
  NTETAPGGPYKVGTSGSCPNVANGNGFFNTMAWVIPKDNNKTAINPVTVEVPYICSEGEDQITVWGFHSD
  DKTQMERLYGDSNPQKFTSSANGVTTHYVSQIGGFPNQTEDEGLKQSGRIVVDYMVQKPGKTGTIVYQRG
  ILLPQKVWCASGRSKVIKGSLPLIGEADCLHEKYGGLNKSKPYYTGEHAKAIGNCPIWVKTPLKLANGTK
  YRPPAKLLKERGFFGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADLKSTQEAINKITKNLNYLSELE
  VKNLQRLSGAMNELHDEILELDEKVDDLRADTISSQIELAVLLSNEGIINSEDEHLLALERKLKKMLGPS
  AVEIGNGCFETKHKCNQTCLDRIAAGTFNAGDFSLPTFDSLNITAASLNDDGLDNHTILLYYSTAASSLA
  VTLMIAIFIVYMVSRDNVSCSICL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_002207
• ORF Size: 1752 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_002207.1, NP_056660
• RefSeq ORF: 1752 bp
• Locus ID: 956538
• MW: 63.3 kDa