BARF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101179

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for BARF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401719


  View other clones from "Virus" (71)

Reconstitution Protocol

USD 330.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Symbol BARF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101179 represents NCBI reference of YP_401719 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGGTTCATCGCACAGCTGCTGCTGTTGGCTTCATGCGTCGCGGCGGGCCAGGCAGTTACCGCTT
TTCTCGGTGAAAGAGTAACATTGACAAGCTATTGGAGGAGAGTGTCATTGGGACCGGAGATCGAGGTCTC
CTGGTTCAAACTTGGACCCGGCGAGGAACAGGTTCTCATTGGGAGAATGCACCATGACGTAATCTTCATC
GAATGGCCATTCAGGGGGTTCTTCGATATCCACCGGAGTGCTAACACCTTTTTTCTCGTGGTGACCGCCG
CCAATATTTCACACGACGGGAATTATCTGTGCCGAATGAAGCTCGGTGAGACCGAGGTGACGAAACAAGA
GCATCTGTCCGTAGTGAAGCCATTGACTCTTTCCGTACACTCAGAACGGTCTCAATTCCCCGATTTCAGC
GTGCTCACTGTAACATGCACCGTAAACGCCTTCCCCCATCCGCATGTTCAGTGGCTGATGCCCGAAGGTG
TCGAACCTGCCCCGACGGCTGCTAATGGTGGAGTGATGAAGGAGAAGGATGGCTCCCTCAGCGTTGCCGT
GGATCTCAGCCTTCCCAAACCATGGCATCTGCCTGTGACCTGCGTGGGCAAAAACGACAAAGAGGAGGCT
CATGGCGTGTATGTCTCAGGATACCTGTCTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101179 representing YP_401719
Red=Cloning sites Green=Tags

MARFIAQLLLLASCVAAGQAVTAFLGERVTLTSYWRRVSLGPEIEVSWFKLGPGEEQVLIGRMHHDVIFI
EWPFRGFFDIHRSANTFFLVVTAANISHDGNYLCRMKLGETEVTKQEHLSVVKPLTLSVHSERSQFPDFS
VLTVTCTVNAFPHPHVQWLMPEGVEPAPTAANGGVMKEKDGSLSVAVDLSLPKPWHLPVTCVGKNDKEEA
HGVYVSGYLSQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_007605
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_007605.1, YP_401719
RefSeq ORF 663 bp
Locus ID 3783772
UniProt ID P03211
MW 24.5 kDa

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.