BALF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101177

Myc-DDK-tagged ORF clone of viral ORF for BALF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401717

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BALF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101177 represents NCBI reference of YP_401717 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCGCACAGACTAGTGAAGACAACCTTGGCTCCCAGTCACAGCCCGGACCATGCGGGTACATCT
  ATTTTTACCCCCTGGCGACCTACCCTCTCAGGGAAGTCGCTACTTTGGGCACAGGCTATGCCGGCCACCG
  GTGCCTGACTGTGCCCCTGCTTTGCGGAATTACCGTAGAGCCAGGTTTCAGTATCAACGTAAAGGCCTTG
  CACAGACGCCCTGACCCGAATTGTGGCCTGCTGCGCGCCACCTCCTATCACCGAGATATATACGTCTTTC
  ACAACGCGCACATGGTTCCACCAATATTCGAAGGACCTGGACTCGAAGCCCTGTGTGGGGAGACGAGAGA
  GGTGTTTGGGTACGACGCATACAGTGCTCTCCCCCGAGAGAGTAGCAAACCCGGTGACTTCTTTCCGGAG
  GGTCTGGATCCCAGTGCGTACCTGGGGGCCGTCGCCATCACCGAGGCTTTCAAAGAGCGGCTGTATAGCG
  GTAATCTGGTCGCCATTCCCAGCCTCAAGCAGGAGGTCGCCGTTGGTCAGTCAGCCTCTGTGCGCGTCCC
  ACTTTACGATAAGGAGGTCTTCCCCGAAGGGGTCCCTCAGCTTAGACAGTTTTATAACTCAGACTTGAGC
  CGGTGCATGCACGAGGCCCTGTATACCGGCCTGGCACAGGCCCTCAGAGTACGCAGAGTTGGTAAATTGG
  TGGAGCTGCTGGAAAAGCAGTCTCTGCAAGACCAGGCCAAGGTCGCCAAGGTGGCTCCCCTGAAGGAATT
  TCCCGCATCAACAATCTCACATCCCGATAGCGGCGCCCTTATGATTGTAGATTCAGCAGCGTGCGAACTG
  GCTGTTTCTTACGCCCCCGCCATGCTCGAGGCGAGCCACGAGACTCCCGCAAGCTTGAACTACGACTCCT
  GGCCCTTGTTTGCCGACTGCGAAGGACCCGAAGCCAGAGTCGCTGCCCTGCACCGGTATAATGCCAGTCT
  GGCCCCACACGTTTCTACGCAAATATTCGCCACCAACAGTGTCTTGTATGTGAGCGGAGTGAGCAAATCT
  ACTGGCCAGGGCAAGGAGAGCTTGTTTAATAGCTTCTATATGACCCATGGACTCGGAACCCTCCAAGAGG
  GTACTTGGGACCCCTGCCGGCGCCCATGCTTCAGCGGATGGGGTGGGCCAGATGTCACTGGAACGAACGG
  CCCCGGCAACTACGCAGTGGAACACTTGGTGTACGCGGCCTCTTTTAGTCCGAACCTTCTCGCTCGATAT
  GCTTATTACCTGCAGTTTTGTCAGGGCCAGAAGAGCAGCCTCACCCCCGTTCCTGAAACCGGATCTTACG
  TTGCAGGGGCCGCAGCCAGCCCGATGTGTTCCCTTTGCGAAGGCCGCGCTCCAGCTGTCTGTCTGAATAC
  ACTGTTCTTTCGACTGCGCGATCGATTCCCGCCCGTGATGTCTACCCAGAGACGGGACCCTTATGTGATT
  AGCGGCGCCTCCGGCTCATATAATGAAACAGATTTCCTCGGCAACTTCTTGAACTTTATCGATAAGGAGG
  ACGACGGCCAGAGGCCGGATGACGAGCCCCGCTATACTTACTGGCAGCTGAACCAAAACCTCCTTGAGCG
  CCTCAGTAGGCTTGGTATCGACGCTGAAGGCAAGCTCGAGAAGGAGCCACACGGTCCGAGAGACTTCGTG
  AAGATGTTCAAGGACGTCGACGCCGCCGTCGATGCAGAGGTCGTGCAGTTCATGAACTCCATGGCTAAAA
  ACAACATAACTTATAAAGACCTGGTTAAGTCTTGCTATCATGTCATGCAGTATAGCTGCAATCCATTTGC
  CCAGCCCGCTTGCCCTATTTTCACACAGCTTTTTTATAGGTCCCTCCTCACAATTCTGCAGGATATCTCT
  CTGCCGATATGCATGTGTTACGAGAACGATAACCCGGGCCTGGGACAAAGTCCGCCTGAGTGGCTGAAGG
  GCCACTACCAAACTCTGTGCACCAATTTCCGCAGCTTGGCCATCGATAAAGGCGTTCTCACGGCCAAAGA
  GGCTAAAGTCGTCCATGGCGAACCCACTTGTGACCTTCCCGACCTGGACGCGGCTTTGCAGGGTAGGGTG
  TACGGTCGGCGGCTGCCCGTTAGGATGTCTAAGGTGTTGATGTTGTGTCCTAGAAACATTAAAATCAAGA
  ATAGAGTTGTGTTCACAGGAGAGAATGCCGCACTGCAAAACAGTTTCATTAAATCAACTACACGCCGGGA
  AAATTACATAATCAATGGCCCTTACATGAAATTTCTCAACACATATCACAAGACCCTTTTTCCGGATACC
  AAGCTGAGCTCTCTCTATCTCTGGCATAATTTCAGTCGCCGGCGGTCTGTGCCAGTACCATCAGGAGCAT
  CCGCTGAAGAGTACAGCGATCTCGCACTGTTTGTTGATGGCGGTAGCAGAGCCCACGAAGAGTCAAATGT
  TATTGACGTGGTGCCTGGTAACCTGGTTACGTACGCGAAACAGCGGCTTAATAACGCCATATTGAAAGCA
  TGTGGCCAAACTCAGTTTTACATTTCCCTCATCCAGGGACTTGTGCCTCGGACTCAATCTGTACCTGCTC
  GCGACTACCCTCATGTGTTGGGGACCAGGGCGGTGGAAAGTGCCGCGGCTTACGCCGAAGCTACTAGCTC
  CCTGACCGCTACTACTGTCGTTTGCGCCGCCACAGACTGTCTCTCACAAGTATGCAAAGCTCGGCCCGTG
  GTTACACTCCCTGTAACAATTAATAAATACACTGGTGTTAACGGGAACAACCAGATATTCCAGGCAGGAA
  ATCTTGGCTACTTCATGGGCAGGGGCGTGGATAGGAACCTGCTGCAAGCTCCAGGTGCAGGTCTGCGAAA
  GCAGGCAGGAGGGAGCTCTATGAGGAAGAAGTTCGTCTTTGCTACACCCACACTGGGGCTGACAGTGAAA
  AGGCGCACACAAGCTGCCACCACCTACGAGATAGAGAATATTCGAGCCGGACTGGAAGCTATTATCTCAC
  AAAAACAGGAGGAGGATTGCGTGTTTGATGTAGTATGCAATCTTGTTGACGCGATGGGGGAGGCTTGTGC
  ATCCCTCACCCGGGACGATGCCGAATACCTGCTGGGAAGGTTTTCCGTGCTTGCCGATTCAGTCCTTGAG
  ACTTTGGCAACCATTGCCTCAAGTGGGATCGAATGGACCGCCGAAGCCGCGCGCGACTTCTTGGAGGGAG
  TGTGGGGCGGACCCGGTGCCGCCCAGGACAACTTCATCAGTGTCGCTGAGCCTGTGTCTACCGCAAGTCA
  AGCTTCTGCTGGACTGCTCCTTGGCGGCGGTGGCCAGGGATCCGGGGGCAGGAGGAAGAGACGCCTGGCC
  ACAGTGCTGCCCGGCCTTGAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101177 representing YP_401717
  Red=Cloning sites Green=Tags
  
  MQGAQTSEDNLGSQSQPGPCGYIYFYPLATYPLREVATLGTGYAGHRCLTVPLLCGITVEPGFSINVKAL
  HRRPDPNCGLLRATSYHRDIYVFHNAHMVPPIFEGPGLEALCGETREVFGYDAYSALPRESSKPGDFFPE
  GLDPSAYLGAVAITEAFKERLYSGNLVAIPSLKQEVAVGQSASVRVPLYDKEVFPEGVPQLRQFYNSDLS
  RCMHEALYTGLAQALRVRRVGKLVELLEKQSLQDQAKVAKVAPLKEFPASTISHPDSGALMIVDSAACEL
  AVSYAPAMLEASHETPASLNYDSWPLFADCEGPEARVAALHRYNASLAPHVSTQIFATNSVLYVSGVSKS
  TGQGKESLFNSFYMTHGLGTLQEGTWDPCRRPCFSGWGGPDVTGTNGPGNYAVEHLVYAASFSPNLLARY
  AYYLQFCQGQKSSLTPVPETGSYVAGAAASPMCSLCEGRAPAVCLNTLFFRLRDRFPPVMSTQRRDPYVI
  SGASGSYNETDFLGNFLNFIDKEDDGQRPDDEPRYTYWQLNQNLLERLSRLGIDAEGKLEKEPHGPRDFV
  KMFKDVDAAVDAEVVQFMNSMAKNNITYKDLVKSCYHVMQYSCNPFAQPACPIFTQLFYRSLLTILQDIS
  LPICMCYENDNPGLGQSPPEWLKGHYQTLCTNFRSLAIDKGVLTAKEAKVVHGEPTCDLPDLDAALQGRV
  YGRRLPVRMSKVLMLCPRNIKIKNRVVFTGENAALQNSFIKSTTRRENYIINGPYMKFLNTYHKTLFPDT
  KLSSLYLWHNFSRRRSVPVPSGASAEEYSDLALFVDGGSRAHEESNVIDVVPGNLVTYAKQRLNNAILKA
  CGQTQFYISLIQGLVPRTQSVPARDYPHVLGTRAVESAAAYAEATSSLTATTVVCAATDCLSQVCKARPV
  VTLPVTINKYTGVNGNNQIFQAGNLGYFMGRGVDRNLLQAPGAGLRKQAGGSSMRKKFVFATPTLGLTVK
  RRTQAATTYEIENIRAGLEAIISQKQEEDCVFDVVCNLVDAMGEACASLTRDDAEYLLGRFSVLADSVLE
  TLATIASSGIEWTAEAARDFLEGVWGGPGAAQDNFISVAEPVSTASQASAGLLLGGGGQGSGGRRKRRLA
  TVLPGLEV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 3384 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401717
• RefSeq ORF: 3384 bp
• Locus ID: 3783678
• UniProt ID: P03211
• MW: 123.1 kDa