LF2 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101168

Myc-DDK-tagged ORF clone of viral ORF for LF2 [Human herpesvirus 4], codon optimized for human cell expression, YP_401708

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101168 represents NCBI reference of YP_401708 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGCCTACCCCGGTGGTGCTCACGCAGCGCTCGCATCCCGCAGAAGCTCATTCCGGAACTCTT
  TGCGGAGGCTTAGACCAACCGAGAAACCTGATACGTCCTTCATGAGAGGCGTGTGGAAGTATGAAATCTT
  TCCTAGTTATGTCAGAGTTACGAACAAGCAGGTGCTCCAGCTCGACGCTCAGTGTCAAGAACTCCCACCA
  TGTCCTTCCGTGGGGCAAATCTTGAGCTTTAAGCTGCCAAGCTTCAGTTTCAATACAACCACCTACGGGT
  CCCGGTATTTTACCGTCGCATTCCTCTTTTTTGGAGCCGAGGACAATGAGGTATTCCTGAAGCCATTCTT
  CGTCATGCACTCAGACCAGGACATCGTGCTTTCCGTGCTGAACCCTCGCTCCCTGTTCATCGAAAAAGGC
  AAGTTCACGTGGTATATCGTCCCAATAAGGCTCGTGAAGAATCCCTATCTGTATCTGCAGATTCTTCCTG
  GTCAATCAGACATACAGCTCACCAGGAGTTGTACTCAGAGTGGCGATAAGCTGAATACGTCAGAACCTCA
  GATATTTCTGAGCGGGTCCCCCGTCACCTCACAGGATGAGTGTCTTCCCTATCTGCTTGCCCAACACACA
  CCCCCTTTTTTGAAATCCTACGCCCGGATTCACACCTTTCCTGGGAAGGTATGTCCCGTGAACGCAATCC
  GGAGGGGAAAGGGATACGTGCGCGTGTCTGTCGATACACCTGACCTGAAAAGAGAAGGCCCACTGAACGT
  CAAGGTTGGTATGACTTTGCTTGACGACGTCATCATAGCGTTTCGGTATAACCCCTACCCTAAGAGCCAT
  TGGAGATGGGACGGGGAATCCACAGATATCCGCTACTTCGGGTCTCCCGTAATCATACCGCCAAACTTTA
  TCACCGAGCTGGAATACAACAACACCTACGAAGCTCCGCTGTCATCCAAGATCACTGCCGTGGTTGTCTC
  CCATTCTTCAAACCCCGTGTTCTATGTCTATCCTCAGGAGTGGAAACCTGGGCAGACACTGAAACTGACC
  GTTAGGAACATTTCCAACAATCCCATCACAATTGTGACCGGACAATCTATGGCCCAGGCTTTTTTCATCT
  ACGCGGGCGACCCTAGCATTTCAACCATCATGAGACGGTACATCCAACGGCAGGGGTGTGCGCTGACACT
  GCCTGGGAATATCGTGGTGGAGAGCTCCAGTCTCCCTACCTTCGAAAGAATAAACAAGACTTTTAACGGC
  AACATTGTGGCGTCAGAAGGTACACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101168 representing YP_401708
  Red=Cloning sites Green=Tags
  
  MAEAYPGGAHAALASRRSSFRNSLRRLRPTEKPDTSFMRGVWKYEIFPSYVRVTNKQVLQLDAQCQELPP
  CPSVGQILSFKLPSFSFNTTTYGSRYFTVAFLFFGAEDNEVFLKPFFVMHSDQDIVLSVLNPRSLFIEKG
  KFTWYIVPIRLVKNPYLYLQILPGQSDIQLTRSCTQSGDKLNTSEPQIFLSGSPVTSQDECLPYLLAQHT
  PPFLKSYARIHTFPGKVCPVNAIRRGKGYVRVSVDTPDLKREGPLNVKVGMTLLDDVIIAFRYNPYPKSH
  WRWDGESTDIRYFGSPVIIPPNFITELEYNNTYEAPLSSKITAVVVSHSSNPVFYVYPQEWKPGQTLKLT
  VRNISNNPITIVTGQSMAQAFFIYAGDPSISTIMRRYIQRQGCALTLPGNIVVESSSLPTFERINKTFNG
  NIVASEGTL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401708
• RefSeq ORF: 1287 bp
• Locus ID: 3783748
• UniProt ID: P03211
• MW: 48.4 kDa