BGLF3 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101149

Myc-DDK-tagged ORF clone of viral ORF for BGLF3 [Human herpesvirus 4], codon optimized for human cell expression, YP_401689

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BGLF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101149 represents NCBI reference of YP_401689 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAACGCGGTGAAAGCGGATATGCCCGATGACCCAATGCTGGCACGGAGGTACGGCCAGTGCCTGG
  AACTGGCCCTGGAGGCCTGCCAAGACACGCCTGAACAGTTTAAACTTGTAGAAACACCACTGAAAAGCTT
  CCTGCTCGTGTCCAACATTCTCCCACAAGATAACCGACCCTGGCATGAAGCTAGATCATCTGGTCGAGTC
  GCTGAAGATGATTACGACTTTTCCAGTCTGGCACTGGAACTGCTGCCCCTTAATCCTCGCCTCCCCGAAG
  AATGGCAGTTTGGAGGTCAGGGCTGGTCAAGCAGGATGGAACCAAGCCAACCCGAAATGGGAATGGGCCT
  TTGCTTTGAGGTGTTTGATGGAGATCTCATGAGGATTGCCCTGGCCTGGAACAAGGACGAGGTTATTGGA
  CAGGCATTGCAGATTCTGGCCCACAGTCAGACATGGACTTCACTGGTGCCCGAGGACCCTCTTCCTTGGA
  TGTGGGCTCTCTTCTACGGACCCCGGTCCCACTGCGAGGAGAGACACTGTGTCTATGCTGCTGCCCGGGG
  GAAAAGGGGGCCAATCCTTCTCCCCACCGCCGTATACACGCCCTGCGCGAACATCGAAGCTTTCCTCGCC
  CACCTGACCCGATGTGTGTACGCCCTCTATCTGGATGTGCGGGACTGGAAAGGTGAGGATATCGCCCCAC
  CCTTCGACGTGTCTCGCCTGAACAAGATGGCCAAACAACTCTGTCTTTTGCCCCAGGAACCATTTTGTAT
  TACTCGGGTCTGCCTTCTTTGTCTCCTGCACAAACAGAATCTGAATGCCCAGTACAAACGGCCCGTAGAC
  ACATATGATCCCTGCCTTATCCTTACTGGCGAAGCTGAAAGGTATATGGTGGATGCCGTCGGGAATTACA
  GGGAAGCATCAACAGGAACCACAGTTCTCTATCCGACATATGATCTGGGCTCTATCGTCGCAGACATGGT
  CACGTATGAGGACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101149 representing YP_401689
  Red=Cloning sites Green=Tags
  
  MFNAVKADMPDDPMLARRYGQCLELALEACQDTPEQFKLVETPLKSFLLVSNILPQDNRPWHEARSSGRV
  AEDDYDFSSLALELLPLNPRLPEEWQFGGQGWSSRMEPSQPEMGMGLCFEVFDGDLMRIALAWNKDEVIG
  QALQILAHSQTWTSLVPEDPLPWMWALFYGPRSHCEERHCVYAAARGKRGPILLPTAVYTPCANIEAFLA
  HLTRCVYALYLDVRDWKGEDIAPPFDVSRLNKMAKQLCLLPQEPFCITRVCLLCLLHKQNLNAQYKRPVD
  TYDPCLILTGEAERYMVDAVGNYREASTGTTVLYPTYDLGSIVADMVTYEDE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 996 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401689
• RefSeq ORF: 996 bp
• Locus ID: 3783703
• UniProt ID: P03211
• MW: 37.7 kDa