BBLF2/BBLF3 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101144

Myc-DDK-tagged ORF clone of viral ORF for BBLF2/BBLF3 [Human herpesvirus 4], codon optimized for human cell expression, YP_401684

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BBLF2/BBLF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101144 represents NCBI reference of YP_401684 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAAACGCCAGCTGAGTCAGTGCGCGCCCGCGTATCATCTGTCACTTTTTATAATGTTACTCAAA
  CAGCCGGCAGGTGGTGGGCCATCTGGGTGGTGGGGATCGTTCCGATTAAGCGGGAGGATGTTGAGACCCT
  CATCGTGGTCCAGGCCTGCCAACCTCCTCTGGGTGGCTCCCTTGAGCCCCCCGTGGTTAATGCACCTAGC
  ACAACTGAACTGAACTTTTTGAGATGGGAACGAGAACTGAGACGGTCAGGCGGCCTCATAGCAATGCTGG
  CTGACGCTGCAGAGAAAGATCTTTTTGACTTGAGTTTTCGGACACGGGACCGGCGGCTGCTCTCCGCTGC
  TAGAGTTGAGGATGAACAGGGCTTGATTTTCCAGCCTCTCTTTCCGGCCCAAGTAGTGTGCCAATCTTGC
  AGTGGCGATGACGGGCGGGATCAACAGCCTCCCCCTGTAGACGGGTTTGGATCAGAGATGGAGGGGGAGC
  AAACGTGCCCGCACGCCCAGAGACACAGTGAGAGTCCTGGCCAGCTTGACGTGTATATTCGCACCCCCCG
  GGGGGACGTGTTTACCTACTCAACCGAAACACCAGATGACCCTTCACCTGTGCCCTTCCGGGACATACTG
  CGCCCAGTAACCTATGAAGTTGACCTGGTCTCTTCAGATGGTGCCACCGGGCGGGGCGGGGATGCCAGAC
  GGCATAGAGTCTCTTTGAAGATTCTGGAGCCAGCTGGCGGTTTCGAATCCTGGTTGGTCAACAGCTGGAG
  CATGGCTGGTGGGGGTCTCTACGCTTTCCTTAGAAGTATTTATGCTTCTTGTTACGCCAACCATAGAGGT
  ACCAAGCCAATATTCTACCTCTTGGACCCGGAGCTGTGTCCTGGGGGATCTGACTTTCAGCCCTATGTGC
  CAGGCTTCCCTTTCCTCCCAATCCACTATGTCGGGCGCGCGAGGCCGGCCTTCTGGCACCGCGCCCCACA
  CTCAGAGGGACTGCTGCTTTTGGATCTGAATCTTGGAGTGAGCGGTACACCCCTTGCGGACGCGCTTCTT
  GGGCTCGATGCACGCTCCGGGCAACGCAGAGGATCTTTGCTTTTGCAGCAGATCTGGCCACCTACTCGCA
  AGGAGATAAATCCCCGGCATGTTTGCACACGCGAGGGGGGGGAGGGCGGTGGAGAAGATGAAACTACCGT
  GGTCGGGCGGGCTGAAGCCACCGCCATCCTCGAAGCCGACGCCACATGGTGGCTGTATGAATTGGCTCGC
  TGTCACCTCAGCGCTCGGGGAGCCCCTGTAGGCACCCCTGATGGGGGGGGACAGGCACGAGATGCTCAGA
  CTTGGCTGAGGGCTCTCCATAGGTATGGTACTTCCGACACACGAAGGGCTCTGGGTGGCCTCTACACAGC
  TGTGACCCGGGTGTTGCTGCATGCGGCAGCCGATCTGGGCCTGACCTGGGCCTACGCGGACGAGTTTATC
  CTTGGCTTCGTGGCCCCTACAAGCGCACATCCTAGTGAAGAACCTCTCGCGCAGGCTTTCCTCCAAGGAG
  TTAAGGACAGCGAGGATGCATCCCGGCTGGATCGAGATGTAATGGGAGGAGAGGCTACCGTCGCTCGCCG
  ACACATCAGGGTCAAGGCGCGACGCGGGCCAGGTTGTCTCCTGATGGCTATCTTCCAGGGCGATTTGTAT
  GTTGGGGGATGTCGCGAGCATTCTGGCCCCTTCCTCGTGTGGCACGAGGCATTCAGCTGGACACTCGACC
  AACTGGCGGCTAGACCCGAGGCCGACAAAGCGCCACCATCCCACGATCATCTGCTCACCTTGGTAAGAGA
  TCTCACCAGGCGCCTCGCTCCCGGAAGACGCCGCAACAGATTTTGGGCACTCCCCCGAGCCTGGCTGCAG
  AGGCTGAGACGGGCTGGTCTGCGACTCTCTGGGAGCCACGTTTGCCTTCTTGACAAGGATGGCGCTCGCC
  CTGCCCCCTGTCAAACCGCGACCGAGCACGGGCTGAGTCCCACGGCATACTTCCGCGAAATCATGGCCTT
  TCTTCTGGACGTTATTAGCGCACTCCATCCGGGGTATACGATCCCCATGGAAATAACCCGCGAAACTGAC
  CTGCTGATGACAGTGTTGTCCCTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101144 representing YP_401684
  Red=Cloning sites Green=Tags
  
  MMETPAESVRARVSSVTFYNVTQTAGRWWAIWVVGIVPIKREDVETLIVVQACQPPLGGSLEPPVVNAPS
  TTELNFLRWERELRRSGGLIAMLADAAEKDLFDLSFRTRDRRLLSAARVEDEQGLIFQPLFPAQVVCQSC
  SGDDGRDQQPPPVDGFGSEMEGEQTCPHAQRHSESPGQLDVYIRTPRGDVFTYSTETPDDPSPVPFRDIL
  RPVTYEVDLVSSDGATGRGGDARRHRVSLKILEPAGGFESWLVNSWSMAGGGLYAFLRSIYASCYANHRG
  TKPIFYLLDPELCPGGSDFQPYVPGFPFLPIHYVGRARPAFWHRAPHSEGLLLLDLNLGVSGTPLADALL
  GLDARSGQRRGSLLLQQIWPPTRKEINPRHVCTREGGEGGGEDETTVVGRAEATAILEADATWWLYELAR
  CHLSARGAPVGTPDGGGQARDAQTWLRALHRYGTSDTRRALGGLYTAVTRVLLHAAADLGLTWAYADEFI
  LGFVAPTSAHPSEEPLAQAFLQGVKDSEDASRLDRDVMGGEATVARRHIRVKARRGPGCLLMAIFQGDLY
  VGGCREHSGPFLVWHEAFSWTLDQLAARPEADKAPPSHDHLLTLVRDLTRRLAPGRRRNRFWALPRAWLQ
  RLRRAGLRLSGSHVCLLDKDGARPAPCQTATEHGLSPTAYFREIMAFLLDVISALHPGYTIPMEITRETD
  LLMTVLSLF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401684
• RefSeq ORF: 2127 bp
• Locus ID: 3783684
• UniProt ID: P03211
• MW: 78.2 kDa