BNRF1 (NC_007605) Virus Tagged ORF Clone

CAT#: VC101095

Myc-DDK-tagged ORF clone of viral ORF for BNRF1 [Human herpesvirus 4], codon optimized for human cell expression, YP_401633

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BNRF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101095 represents NCBI reference of YP_401633 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCGCGGGAGGGAGACCCAGATGCCGGTGGCCCGATACGGCGGCCCGTTCATTATGGTGCGGC
  TGTTCGGGCAAGATGGTGAGGCAAACATTCAAGAGGAGAGACTGTACGAGCTGTTGTCTGACCCCCGCTC
  CGCCCTGGGATTGGACCCCGGGCCTCTTATAGCCGAGAATCTCTTGTTGGTAGCTCTGCGCGGCACCAAC
  AACGACCCAAGGCCTCAGAGGCAGGAGCGGGCCAGGGAACTGGCACTTGTGGGAATATTGCTGGGTAATG
  GAGAACAGGGTGAACACCTTGGCACAGAAAGCGCCCTGGAGGCTAGCGGCAACAACTATGTGTACGCTTA
  TGGGCCTGATTGGATGGCTCGGCCATCAACATGGTCCGCCGAAATACAGCAATTCCTTAGACTTCTTGGG
  GCGACCTACGTGCTGAGGGTGGAGATGGGTCGGCAGTTCGGCTTCGAGGTTCACCGGTCCAGACCTAGCT
  TTAGACAATTCCAGGCCATCAACCATCTGGTGCTCTTCGACAATGCCCTGAGAAAGTACGACAGCGGACA
  GGTCGCTGCTGGGTTTCAACGCGCTCTTCTCGTGGCCGGGCCTGAGACCGCTGACACCCGACCGGACCTC
  AGAAAATTGAATGAATGGGTGTTTGGCGGGAGAGCCGCCGGGGGTAGGCAGCTGGCCGACGAACTTAAGA
  TCGTAAGCGCTCTTCGAGATACATACAGCGGCCACCTTGTACTGCAGCCAACAGAAACGCTGGACACTTG
  GAAAGTCCTGTCTCGGGATACGCGGACTGCACATAGCTTGGAGCACGGCTTCATCCACGCCGCTGGGACT
  ATACAGGCAAACTGTCCACAACTGTTCATGAGACGCCAACATCCAGGCCTGTTTCCTTTCGTGAACGCCA
  TCGCTAGTAGCCTGGGATGGTACTACCAGACTGCAACAGGTCCTGGCGCAGATGCAAGGGCCGCTGCCCG
  GAGGCAACAAGCCTTCCAGACCCGGGCTGCAGCAGAGTGCCACGCAAAGAGCGGGGTCCCTGTCGTGGCA
  GGTTTCTATAGGACAATCAACGCTACTTTGAAGGGAGGCGAGGGCCTTCAGCCCACGATGTTTAATGGTG
  AGCTGGGGGCCATTAAGCATCAGGCACTCGATACGGTGAGGTACGACTACGGACACTACCTGATTATGCT
  CGGCCCATTCCAGCCATGGTCAGGGCTCACTGCCCCTCCCTGTCCTTACGCAGAATCATCTTGGGCTCAG
  GCCGCTGTCCAGACAGCGCTGGAACTGTTTTCCGCCCTGTACCCCGCTCCCTGTATCTCTGGATACGCCC
  GGCCTCCAGGCCCTTCTGCCGTGATCGAGCATCTCGGAAGTCTCGTCCCCAAGGGCGGGCTCCTGCTGTT
  CCTTAGCCATCTCCCCGATGACGTGAAGGACGGACTGGGCGAGATGGGACCTGCCAGGGCTACCGGTCCG
  GGCATGCAGCAGTTTGTCTCATCCTATTTTCTCAACCCTGCATGTAGCAATGTCTTTATTACAGTGCGGC
  AGCGCGGGGAGAAGATCAACGGACGCACTGTGCTGCAGGCCCTGGGGCGAGCCTGCGACATGGCTGGATG
  CCAGCACTATGTACTGGGATCTACTGTTCCCCTCGGGGGCCTGAACTTCGTGAACGACCTGGCCAGCCCT
  GTGAGCACAGCCGAGATGATGGATGACTTCTCTCCATTCTTTACCGTCGAGTTTCCTCCAATACAAGAAG
  AGGGCGCATCATCACCTGTTCCACTGGATGTGGATGAGTCAATGGACATAAGTCCAAGTTATGAACTGCC
  TTGGCTGAGCCTGGAATCTTGCCTGACATCAATTCTGTCTCACCCAACCGTGGGATCTAAGGAACATTTG
  GTTCGACACACCGATCGCGTGTCAGGTGGCCGGGTAGCTCAGCAGCCCGGCGTAGGGCCACTGGACTTGC
  CCCTTGCAGACTACGCATTTGTGGCTCATAGTCAGGTGTGGACCCGCCCTGGAGGTGCACCACCACTTCC
  ATATCGGACATGGGACCGAATGACTGAAAAGCTGCTCGTCAGCGCTAAGCCCGGAGGCGAAAATGTGAAA
  GTGAGCGGCACTGTGATCACTCTGGGAGAGCAAGGGTATAAAGTTAGCCTGGATCTCCGCGAGGGAACGA
  GACTCGCCATGGCCGAAGCCCTTTTGAACGCTGCATGTGCTCCAATATTGGATCCGGAAGACGTACTTCT
  CACACTGCATCTGCATCTCGACCCCAGGCGCGCCGATAACAGCGCCGTGATGGAGGCGATGACCGCTGCC
  AGCGATTATGCAAGGGGTCTTGGTGTTAAGCTGACGTTTGGATCAGCCTCCTGTCCTGAGACCGGGAGCT
  CTGCCTCTAACTTCATGACCGTGGTGGCCTCCGTGTCAGCTCCCGGAGAATTCTCTGGCCCACTGATTAC
  ACCTGTGTTGCAGAAGACCGGGTCCCTTCTGATTGCTGTGCGGTGTGGTGATGGGAAAATCCAGGGTGGG
  TCACTTTTCGAGCAGCTTTTCTCTGACGTCGCCACCACTCCCCGAGCCCCCGAGGCCCTGAGCCTGAAAA
  ACCTGTTCAGGGCCGTGCAGCAGCTCGTTAAGAGTGGGATCGTACTCAGCGGGCACGACATTTCAGACGG
  AGGACTGGTCACCTGCCTGGTAGAGATGGCTCTGGCAGGCCAGCGCGGCGTAACCATCACCATGCCCGTC
  GCCTCAGATTACCTGCCCGAGATGTTCGCCGAACACCCCGGCCTGGTGTTCGAAGTGGAAGAAAGATCCG
  TGGGAGAGGTACTGCAGACTCTGCGGTCCATGAACATGTACCCCGCTGTGCTGGGGCGCGTGGGAGAACA
  GGGACCCGACCAGATGTTCGAAGTCCAGCACGGCCCGGAGACAGTCCTGCGACAGAGCCTGCGGCTGCTG
  CTCGGAACTTGGAGCTCATTTGCATCCGAGCAGTATGAATGCCTGAGACCTGACCGAATCAACCGGAGCA
  TGCATGTAAGTGACTATGGCTACAACGAGGCCTTGGCTGTTTCACCGCTTACTGGCAAGAACCTCTCTCC
  GCGGAGGTTGGTGACAGAGCCCGACCCAAGGTGTCAGGTGGCGGTACTGTGCGCCCCTGGAACTAGGGGC
  CATGAAAGTCTGCTGGCAGCTTTTACTAACGCAGGATGCCTTTGTAGACGGGTGTTCTTCCGCGAAGTCA
  GAGATAATACCTTTCTGGATAAGTACGTGGGACTGGCCATAGGTGGAGTGCACGGGGCTAGAGACTCAGC
  TCTCGCGGGGCGAGCTACAGTGGCACTGATAAATCGCTTCCCTGCCCTCAGAGACGCTATACTGAAGTTC
  CTGAATAGACCAGATACTTTTAGTGTGGCACTCGGTGAACTGGGCGTGCAGGTTCTGGCCGGCCTCGGCG
  CAGTCGGGTCAACGGATAACCCACCTGCTCCAGGAGTGGAGGTTAACGTCCAGAGGTCACCCCTTATCCT
  CGCTCCCAACGCTAGCGGAATGTTCGAGTCCCGGTGGCTGAACATTAGTATTCCAGCCACAACTAGCAGC
  GTGATGTTGAGGGGACTGAGAGGATGCGTCCTGCCATGTTGGGTGCAGGGCTCCTGCCTCGGTCTCCAGT
  TCACAAATCTGGGCATGCCGTACGTGCTGCAGAACGCCCACCAAATAGCATGTCACTTTCACTCCAACGG
  CACTGACGCATGGCGGTTCGCCATGAACTACCCACGAAACCCTACAGAGCAAGGAAATATTGCTGGCCTC
  TGCTCACGGGATGGTCGCCATCTTGCCCTCTTGTGTGACCCCAGCCTCTGCACGGACTTTTGGCAGTGGG
  AACACATCCCGCCCGCGTTCGGACATCCAACCGGGTGTTCACCGTGGACGCTTATGTTTCAAGCTGCTCA
  CCTGTGGTCCCTCCGCCATGGTCGGCCATCAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101095 representing YP_401633
  Red=Cloning sites Green=Tags
  
  MEERGRETQMPVARYGGPFIMVRLFGQDGEANIQEERLYELLSDPRSALGLDPGPLIAENLLLVALRGTN
  NDPRPQRQERARELALVGILLGNGEQGEHLGTESALEASGNNYVYAYGPDWMARPSTWSAEIQQFLRLLG
  ATYVLRVEMGRQFGFEVHRSRPSFRQFQAINHLVLFDNALRKYDSGQVAAGFQRALLVAGPETADTRPDL
  RKLNEWVFGGRAAGGRQLADELKIVSALRDTYSGHLVLQPTETLDTWKVLSRDTRTAHSLEHGFIHAAGT
  IQANCPQLFMRRQHPGLFPFVNAIASSLGWYYQTATGPGADARAAARRQQAFQTRAAAECHAKSGVPVVA
  GFYRTINATLKGGEGLQPTMFNGELGAIKHQALDTVRYDYGHYLIMLGPFQPWSGLTAPPCPYAESSWAQ
  AAVQTALELFSALYPAPCISGYARPPGPSAVIEHLGSLVPKGGLLLFLSHLPDDVKDGLGEMGPARATGP
  GMQQFVSSYFLNPACSNVFITVRQRGEKINGRTVLQALGRACDMAGCQHYVLGSTVPLGGLNFVNDLASP
  VSTAEMMDDFSPFFTVEFPPIQEEGASSPVPLDVDESMDISPSYELPWLSLESCLTSILSHPTVGSKEHL
  VRHTDRVSGGRVAQQPGVGPLDLPLADYAFVAHSQVWTRPGGAPPLPYRTWDRMTEKLLVSAKPGGENVK
  VSGTVITLGEQGYKVSLDLREGTRLAMAEALLNAACAPILDPEDVLLTLHLHLDPRRADNSAVMEAMTAA
  SDYARGLGVKLTFGSASCPETGSSASNFMTVVASVSAPGEFSGPLITPVLQKTGSLLIAVRCGDGKIQGG
  SLFEQLFSDVATTPRAPEALSLKNLFRAVQQLVKSGIVLSGHDISDGGLVTCLVEMALAGQRGVTITMPV
  ASDYLPEMFAEHPGLVFEVEERSVGEVLQTLRSMNMYPAVLGRVGEQGPDQMFEVQHGPETVLRQSLRLL
  LGTWSSFASEQYECLRPDRINRSMHVSDYGYNEALAVSPLTGKNLSPRRLVTEPDPRCQVAVLCAPGTRG
  HESLLAAFTNAGCLCRRVFFREVRDNTFLDKYVGLAIGGVHGARDSALAGRATVALINRFPALRDAILKF
  LNRPDTFSVALGELGVQVLAGLGAVGSTDNPPAPGVEVNVQRSPLILAPNASGMFESRWLNISIPATTSS
  VMLRGLRGCVLPCWVQGSCLGLQFTNLGMPYVLQNAHQIACHFHSNGTDAWRFAMNYPRNPTEQGNIAGL
  CSRDGRHLALLCDPSLCTDFWQWEHIPPAFGHPTGCSPWTLMFQAAHLWSLRHGRPSE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007605
• ORF Size: 3954 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_007605.1, YP_401633
• RefSeq ORF: 3954 bp
• Locus ID: 3783722
• UniProt ID: P03211
• MW: 142.8 kDa