LF1 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101081

Myc-DDK-tagged ORF clone of viral ORF for LF1 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129505

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101081 represents NCBI reference of YP_001129505 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTGCAAACGGACACACATGCCTGGCGCGTTGAAATCGGCACCCGGGGTCTGATGTTCTCAAACT
  GCGTGCCATTGCATCTTCCTGAGGGCCAGTATCACAAGCTGAGACTTCCAGTGTCTGCGTACGAAGCCCT
  GGCTGTAGCTCGCTATGGACTGGTCGGCAGCCTCTGGGAGGTCCCCGCTGTGAACAGTGCACTCCAATGC
  CTGGCCGCTGCCGCACCATGTAAGGACGTCAAAATCTACCCTTCTTGCATTTTTCAGGTCCACGCCCCTA
  TGTTTGTTACCATCAAAACCAGTCTTCGGTGCTTGAATCCTCATGACCTGTGCTTGTGCCTGATCTGCGT
  TGGTGCAGCTATCCTGGACATTCCTCTGCTTTGCGCTCCACGGGATGGAGCGGGCGCCCGGGCTGCAGAA
  GGTCAAGCAGCCGCAGCCCAGGGTGGCAAGCTTCGGGTTTGGGGCAGATTGTCCCCCAGCAGCCCCATCT
  CTCTCTCTCTGGCGTTTCCATACGCTGGCCCTCCTCCCGTGGCCTGGTACAGACATAGTATCAAGCTGAC
  AAGGTCTGAGGGAGTGGGGATTGGTAAAGACTGTGCTCAGGATCACGCTTGCCCTGTCCCTCCACAGGGA
  CATGCGTCTTCCGCCGCCGATCAGGCTGGTGTGCCCGAGAGAGGGCGGAAGAGAGCACATGAGGGACCTG
  GTGCCGGAGAAGCAGCAAGTGCAGGCCGCGGAGACGTCGCCTTGAGTCAGAGCCGCGCTTTGCTCTGGAG
  GGGTCTTGGTTGGGATACAGGGAGAGGACGGCTGGCACCTGGCCTGGCAATGTCTCGGGATGCCGCAAGT
  GGCAGTGTCCACCTTGACATACAGGTGGACAGGGCCGAAGAGGGGTGGGTGTGTGATGTGCTGCTCGAAC
  CCGGTCCGCCCACAGCTAGGGAGGGATGCTTCCTCTCAATGGATCCCGGCCTGGTGACTCTGAAAGACGC
  ATGGACCCTGTTCCCTCTCCACCCTGAGCATGACGCAGTCGTGCCTCCTAAGGAGGAGATACATGTTATG
  GCCCAGGGCCACCTGCAGGGGGGCACCCCCTCTCTTTGGGGCTTTACATTCCAGGAGGCTGCCTGCGATC
  AATGGGTCCTCAGGCCCCGCGTGTGGACTGCCCACTCACCCATTAAGATGACTGTCTACAACTGCGGGCA
  CAAACCATTGCACATCGGTCCATCAACTCGCCTGGGTCTCGCCCTCTTCTGGCCCGCTGAGCGGTCAGAC
  AACTTGGATGCTGGCCGGATCTTTTATCAGCTGACAAGCGGTGAACTTTACTGGGGACGGACCGTCGCAC
  GCCCCCCAACCCTCACGCTGCCAGTTGATGAACTGAGACCTTGGCCAAAGTTGACGCCCGAAGAACCCAT
  GCAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101081 representing YP_001129505
  Red=Cloning sites Green=Tags
  
  MALQTDTHAWRVEIGTRGLMFSNCVPLHLPEGQYHKLRLPVSAYEALAVARYGLVGSLWEVPAVNSALQC
  LAAAAPCKDVKIYPSCIFQVHAPMFVTIKTSLRCLNPHDLCLCLICVGAAILDIPLLCAPRDGAGARAAE
  GQAAAAQGGKLRVWGRLSPSSPISLSLAFPYAGPPPVAWYRHSIKLTRSEGVGIGKDCAQDHACPVPPQG
  HASSAADQAGVPERGRKRAHEGPGAGEAASAGRGDVALSQSRALLWRGLGWDTGRGRLAPGLAMSRDAAS
  GSVHLDIQVDRAEEGWVCDVLLEPGPPTAREGCFLSMDPGLVTLKDAWTLFPLHPEHDAVVPPKEEIHVM
  AQGHLQGGTPSLWGFTFQEAACDQWVLRPRVWTAHSPIKMTVYNCGHKPLHIGPSTRLGLALFWPAERSD
  NLDAGRIFYQLTSGELYWGRTVARPPTLTLPVDELRPWPKLTPEEPMQH
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129505
• RefSeq ORF: 1407 bp
• Locus ID: 5176164
• MW: 50.6 kDa