helicase-primase subunit BBLF2/3 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101055

Myc-DDK-tagged ORF clone of viral ORF for BBLF2/BBLF3 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129478

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: helicase-primase subunit BBLF2/3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101055 represents NCBI reference of YP_001129478 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGACACCAGCGGAGTCAGTGAGAGCACGGGTCTCTAGTGTTACCTTCTATAACGTGACACAAA
  CCGCAGGCCGGTGGTGGGCCATTTGGGTTGTGGGGATCGTGCCGATTAAACGAGAGGACGTTGAGACTCT
  GATTGTTGTGCAGGCATGCCAGCCACCACTGGGAGGGAGTCTGGAACCCCCAGTGGTCAACGCGCCCAGC
  ACAACCGAGCTGAATTTTCTGCGGTGGGAGAGGGAACTGCGCAGGTCCGGAGGACTGATCGCCATGCTGG
  CTGACGCTGCTGAGAAAGACCTCTTTGATCTCTCCTTCAGAACTCGGGATCGGCGATTGCTCAGCGCCGC
  TAGAGTTGAGGATGAGCAGGGCCTGATTTTCCAGCCACTGTTCCCCGCCCAGGTCGTGTGCCAGAGCTGC
  AGTGGGGATGATGGCCGCGACCAACAGCCACCTCCAGTGGACGGATTCGGCAGCGAAATGGAGGGAGAGC
  AGACTTGTCCCCACGCACAGAGACACTCTGAATCACCTGGCCAGCTTGATGTGTATATCCGGACCCCACG
  CGGAGACGTTTTTACATATAGTACCGAAACCCCAGATGATCCCTCCCCTGTACCCTTTCGGGATATCCTG
  AGGCCTGTTACATATGAAGTGGACCTGGTGAGCTCTGACGGTGCAACAGGGCGGGGGGGAGACGCTCGAC
  GCCACAGAGTCTCCCTTAAGATCCTCGAGCCCGCCGGAGGCTTTGAGAGCTGGCTTGTCAACTCATGGAG
  CATGGCAGGTGGAGGCTTGTATGCCTTTCTTAGATCCATCTATGCCTCATGCTACGCCAACCACCGCGGA
  ACAAAACCGATTTTCTATCTCCTTGATCCTGAACTCTGCCCCGGTGGCAGCGACTTCCAGCCATACGTCC
  CGGGATTCCCCTTTCTTCCTATACACTACGTGGGCCGCGCTCGCCCTGCCTTTTGGCATCGCGCACCTCA
  CTCAGAGGGTCTGCTGCTGCTGGATCTGAATCTGGGCGTGAGCGGTACTCCCCTTGCCGACGCACTGCTG
  GGGCTGGACGCGCGCAGCGGACAGCGCCGCGGATCTCTCCTCCTGCAGCAAATCTGGCCTCCTACCCGAA
  AGGAAATAAACCCCAGGCATGTTTGCACCCGGGAAGGCGGTGAAGGTGGTGGCGAAGACGAGACGACCGT
  AGTGGGCAGGGCCGAGGCCACGGCAATTTTGGAAGCAGATGCAACATGGTGGCTGTATGAACTGGCACGG
  TGTCATCTGAGCGCGAGAGGAGCCCCCGTGGGGACACCCGATGGTGGGGGACAGGCCCGCGACGCTCAGA
  CATGGCTCAGAGCCCTCCACAGATATGGGACATCCGACACTCGGAGAGCGCTCGGGGGATTGTATACCGC
  AGTGACTCGGGTACTGTTGCACGCGGCCGCGGACCTCGGCCTGACATGGGCATATGCCGATGAGTTTATC
  CTGGGATTTGTGGCTCCAACCTCTGCTCACCCATCTGAGGAGCCCCTCGCTCAGGCCTTCCTGCAGGGGG
  TGAAGGATTCTGAGGACGCAAGCCGGCTGGATAGGGACGTAATGGGAGGCGAAGCTACCGTCGCAAGGCG
  CCACATCCGAGTCAAAGCTCGAAGAGGCCCTGGGTGTCTCTTGATGGCCATCTTCCAAGGAGATCTGTAC
  GTGGGCGGGTGTCGCGAGCACTCCGGTCCCTTCCTGGTATGGCACGAGGCATTTTCTTGGACGCTGGATC
  AGCTGGCCGCGCGGCCCGAGGCAGATAAGGCCCCTCCATCTCATGACCATCTGCTGACGCTTGTTAGAGA
  CCTGACTAGACGCCTGGCGCCAGGACGGAGGCGCAATCGATTTTGGGCGCTGCCTCGGGCTTGGCTGCAG
  CGGCTGCGGAGAGCAGGACTGAGGCTGAGTGGCTCACATGTCTGCCTTCTGGACAAAGATGGAGCCCGCC
  CCGCACCATGCCAGACTGCGACTGAGCACGGCTTGTCCCCTACCGCATATTTCCGGGAGATCATGGCCTT
  TCTGCTGGATGTAATCAGCGCTCTGCACCCTGGCTATACAATTCCTATGGAGATTACTAGAGAGACAGAC
  CTGCTCATGACAGTGCTGTCACTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101055 representing YP_001129478
  Red=Cloning sites Green=Tags
  
  MMETPAESVRARVSSVTFYNVTQTAGRWWAIWVVGIVPIKREDVETLIVVQACQPPLGGSLEPPVVNAPS
  TTELNFLRWERELRRSGGLIAMLADAAEKDLFDLSFRTRDRRLLSAARVEDEQGLIFQPLFPAQVVCQSC
  SGDDGRDQQPPPVDGFGSEMEGEQTCPHAQRHSESPGQLDVYIRTPRGDVFTYSTETPDDPSPVPFRDIL
  RPVTYEVDLVSSDGATGRGGDARRHRVSLKILEPAGGFESWLVNSWSMAGGGLYAFLRSIYASCYANHRG
  TKPIFYLLDPELCPGGSDFQPYVPGFPFLPIHYVGRARPAFWHRAPHSEGLLLLDLNLGVSGTPLADALL
  GLDARSGQRRGSLLLQQIWPPTRKEINPRHVCTREGGEGGGEDETTVVGRAEATAILEADATWWLYELAR
  CHLSARGAPVGTPDGGGQARDAQTWLRALHRYGTSDTRRALGGLYTAVTRVLLHAAADLGLTWAYADEFI
  LGFVAPTSAHPSEEPLAQAFLQGVKDSEDASRLDRDVMGGEATVARRHIRVKARRGPGCLLMAIFQGDLY
  VGGCREHSGPFLVWHEAFSWTLDQLAARPEADKAPPSHDHLLTLVRDLTRRLAPGRRRNRFWALPRAWLQ
  RLRRAGLRLSGSHVCLLDKDGARPAPCQTATEHGLSPTAYFREIMAFLLDVISALHPGYTIPMEITRETD
  LLMTVLSLF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129478
• RefSeq ORF: 2127 bp
• Locus ID: 5176202
• MW: 78.2 kDa