helicase-primase subunit BBLF4 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101052

Myc-DDK-tagged ORF clone of viral ORF for BBLF4 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129475

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: helicase-primase subunit BBLF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101052 represents NCBI reference of YP_001129475 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGAGCCAAGGGCTCCCGAGGCCCTGTCAAGCACTTTCATGCTGAACATGACATCAGACGCCT
  CTGTTCGCCGAATCGTTCGGCGAATCGGCACACTGGCCAAGCGGAGAGTGCAGCAGCTGCCAGACATGGA
  AACCTTCTCACCCGAGTTTGATCCCGAAATTTCCGAGCCTCCCTTCCTGCCATTTTCCGCCTACGTGATT
  ACTGGTACAGCCGGCGCTGGGAAGTCCACTTCTGTGTCATGCCTCCATCATACAATGGATTGTTTGGTGA
  CAGGAGCCACAACCGTCGCGGCGCAGAACCTCAGTCAAACCCTCAGAGCGTACTGCCCGACAGTGTACAG
  CGCGTTTGGCTTTAAAAGCCGCCACATCAACATGACACAGAGAGTTTCCTCACATGGTAGGAGCACCGAC
  GCCGCTCTTGAAGAACTTCAGCGCCGCGACATGGCTAAGTATTGGCCAGTGTTGTCAGATATCGCAGCCG
  AGTTTCGCCGGACAAAGCCAAGGGGACTTTATTCTGGTGTGTCCGGGCCTGCATTCGAAGTGTTGAGGGA
  TATGCACCAAGGCCAATTGTGGACAACGAATGTGATCGTGGTGGACGAGGCAGGAACTCTGTCCGTACAT
  ATCCTGACCGCTGTTGTTTTTTGCTATTGGTTTTTCAATGCATGGCTCAGAACCCCTCTCTACAGGAGAG
  GACGGATCCCTTGCATCGTGTGCGTTGGTTCACCCACCCAAACAGATGCCTTCCAATCTAGCTTTAGCCA
  TGAAACACAAGTGAATAAGATAAGAGAATGTGACAACATTCTTACTTTCCTTGTGGGCAACCCTAGAGCC
  GCCACTTACGTCGACGTAGCACGGAACTGGGCATTGTTTATAAATAACAAACGGTGTACCGACGTGCAAT
  TCGGACACCTGATGAAAACCCTGGAATACGGACTCGAGCTGTCTCCAGATATCCTGGCGTACGTGGATCG
  CTTCGTAGTTCCACGCGCGGCTATCATGGATCCAACACAGTACGTGGGATGGACACGGCTTTTCCTTTCC
  CATGCCGAAGTGAAGACTTTCCTCACCACTCTCCACGCTACACTCAAGACCGCCGGTCAGGGCAGAGCCG
  CTAGGGGAACCGGGGGAGACGGAGGAGGGGTGACAATGTTTACTTGTCCCGTAGAGTGCGAGGTTTTTCT
  TGAGCCCTTGGCTCAATATAAGACCCTGGTAGGACTGCCTGGTCTTACAGCTCACACATGGCTGCAGAAA
  AATTATGCACGCCTGGGCAACTATAGTCAATTTGCCGACCAGGATATGGTCCCTGTGGGGACAGAACAGG
  ATGAAGAGAGGGTTAAGGTGACCTATAATGTGACTTACGTTAAGCACTCAAGCGTGAGTGTAAACTGCAA
  AACCAAGAAGAGCATTTGCGGATACACGGGCACCTTCGGGGACTTCATGGACACACTTGAGGCTGATTCC
  TTCGTGGAGGCACACGGGCATGAGCAACCTGAGTATGTGTATTCATTTCTCGCCAGGCTTATCTATGGTG
  GAATCTACGCATTTAGCCACGGAGGACATTCCCTCTGTGAAAACGGGGAATACGTGGCTGAGCTTGGTGC
  CGTGCCGCTGCCAGGTAGGACCTGGGACCCCGAAGTTACGGCTGGCATGGAGCTCGGAGAGTTGCCTCTG
  GAGGTCGCCTGGGATGGAGAGCGCAGTCCTGCTGCCGTGTTCTACGCGAGGGTGCTGGCACCCCCAGCCG
  CCAACAGCGCTCCCCTGTGCTCACTGCTGAATATATACAACGACCTGAGGGCTTATTTTCGCCAGTGCCT
  TGATGTCGCCGTTAGGTATGGGCGCCGCGAGTTCCGCGACCTTCCGTTTTGCACCTTCACCAATAATATG
  CTCATTAGAGATAATATCGAGTTCACCAGCGACGAGCCGCTGTTGCATGGGCTCTTGGACTATGCCTCCA
  CTACCGAGAACTATACACTGCTCGGCTATACCCACCTGAACGTTTTTTTTGGAATTAGAGGCAAACAACA
  GCCTCAAGACGCAGGCTCATCAAGAATGCCGAGACTGATGGTCAAGGATGAAGCAGGGTTTGTGTGCTGC
  CTGGAACACAATACTAATAAACTGTACGAGACAATCGAGGACAAAAGTCTGAACTTGTGCTCAATACGAG
  ACTACGGGATAAGCAGTAAGCTTGCTATGACGATCGCTAAGGCTCAAGGGCTGTCCCTTAACAAGGTTGC
  CATATGTTTTGGCTCCCATAGAAACATTAAGCCTGGGCATGTTTACGTTGCACTTAGCCGGGCGCGCCAT
  TCCAACTGCGTTGTAATGGATAGAAACCCCCTGTCTGAAATGATAACCGGAGAGGGAAACCCAGCAAGCG
  GATATATTGTTGATGCCCTGAAAAATTCCCGGGCTTTGCTGGTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101052 representing YP_001129475
  Red=Cloning sites Green=Tags
  
  MAEEPRAPEALSSTFMLNMTSDASVRRIVRRIGTLAKRRVQQLPDMETFSPEFDPEISEPPFLPFSAYVI
  TGTAGAGKSTSVSCLHHTMDCLVTGATTVAAQNLSQTLRAYCPTVYSAFGFKSRHINMTQRVSSHGRSTD
  AALEELQRRDMAKYWPVLSDIAAEFRRTKPRGLYSGVSGPAFEVLRDMHQGQLWTTNVIVVDEAGTLSVH
  ILTAVVFCYWFFNAWLRTPLYRRGRIPCIVCVGSPTQTDAFQSSFSHETQVNKIRECDNILTFLVGNPRA
  ATYVDVARNWALFINNKRCTDVQFGHLMKTLEYGLELSPDILAYVDRFVVPRAAIMDPTQYVGWTRLFLS
  HAEVKTFLTTLHATLKTAGQGRAARGTGGDGGGVTMFTCPVECEVFLEPLAQYKTLVGLPGLTAHTWLQK
  NYARLGNYSQFADQDMVPVGTEQDEERVKVTYNVTYVKHSSVSVNCKTKKSICGYTGTFGDFMDTLEADS
  FVEAHGHEQPEYVYSFLARLIYGGIYAFSHGGHSLCENGEYVAELGAVPLPGRTWDPEVTAGMELGELPL
  EVAWDGERSPAAVFYARVLAPPAANSAPLCSLLNIYNDLRAYFRQCLDVAVRYGRREFRDLPFCTFTNNM
  LIRDNIEFTSDEPLLHGLLDYASTTENYTLLGYTHLNVFFGIRGKQQPQDAGSSRMPRLMVKDEAGFVCC
  LEHNTNKLYETIEDKSLNLCSIRDYGISSKLAMTIAKAQGLSLNKVAICFGSHRNIKPGHVYVALSRARH
  SNCVVMDRNPLSEMITGEGNPASGYIVDALKNSRALLVY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 2427 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129475
• RefSeq ORF: 2427 bp
• Locus ID: 5176217
• MW: 90.0 kDa