EBNA-2 (NC_009334) Virus Tagged ORF Clone

CAT#: VC101019

Myc-DDK-tagged ORF clone of viral ORF for EBNA-2 [Human herpesvirus 4 type 2], codon optimized for human cell expression, YP_001129441

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBNA-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101019 represents NCBI reference of YP_001129441 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAACTTATTACTTGGCTCTGCACGGAGGTCAATCTTATAATCTCATTGTGGACACGGATATGAGCG
  GGAACCCTTCCCTGAGCGTGATTCCTACAAATCCCTACCAGGAGCAGCTGTCTAACAACCCGCTGATCCA
  ACTTCAGATTGTGGTTGGGGAGAATACAGGAGCTCCAGCCCCGCCTCAGCCTCCGCCACCCCCACCCCCC
  CCACCTCCACCAGAGAGACGAGACGCTTGGACGCAGGAACCCTTGCCTCTTGACATGAATCCCCTGGGTA
  GCGACGCTAGTCAGGGACCTCTCGCATCCAGCATTAGAATGCTTTGCATGGCCCAGTACTTGCTCCGCAA
  CGCTAGAGGACAACAGGGTCTTCTTAGACCCCTGGGTCCTCAGACCCGGTCCCAGGTGACCCTCGAGAGG
  CAGCCCGTGCACAACCCAAGGCAGGAGGCTCCAATCATCCTTCTGCAGAGTCCTGCACCGCCACGCTTCA
  CCCCCGTGCCGATGGTCGCTCTGGGACACACCCTTCAACCTACCCCCCCTCCCCGCCCTACTCTGCCCCA
  GCCCAGAATTCCGTTGATCATCCCCCCACGACACACCAATCAGCCTGCAACCACACCCCCCACGGCCCCT
  CAGCGCCTTACATTGGGGCACCAGTTGAGTCTGCCTCCGCATCCGCCCCCACACCAGTCTACACCTCATT
  GCTCTAGTGACAGTACAGGATTGCCACCACCTCCTACAAGTTACTCAATTCCTTCTATGACTCTGTCTCC
  AGAACCTTTGCCACCGCCCGCAGCCCCAGCTCACCCACTGCCTGGAGTCATTTATGACCAGCAAGCCCTT
  CCACCTACCCCTGGTCCCCCCTGGTGGCCTCCCGTGCGCGACCCAACCCCAACTACACAGACACCCCCAA
  CAAACACTAAGCAGGGACCAGATCAGGGCCAGGGAAGGGGGAGATGGCGCGGGAGGGGCAGGTCCAAGGG
  CCGGGGCAGAATGCATAAGTTGCCTGAACCACGGAGGCCCGGCCCTGATACGTCTAGCCCCAGCATGCCT
  CAGCTGTCTCCAGTTGTCAGTCTCCACCAGGGGCAAGGCCCAGAGAATTCTCCAACTCCCGGCCCCTCCA
  CGGCTGGCCCAGTCTGCCGGGTGACCCCCTCAGCCACCCCAGATATCTCTCCAATACACGAACCCGAATC
  CTCTGATTCTGAGGAACCGCCTTTCCTCTTTCCCTCTGATTGGTATCCGCCTACCCTTGAACCTGCCGAG
  CTCGATGAAAGCTGGGAGGGCATCTTCGAAACCACGGAGTCCCATTCCTCAGATGAGGAAAATGTGGGCG
  GTCCTAGCAAAAGACCCAGGACAAGCACTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101019 representing YP_001129441
  Red=Cloning sites Green=Tags
  
  MPTYYLALHGGQSYNLIVDTDMSGNPSLSVIPTNPYQEQLSNNPLIQLQIVVGENTGAPAPPQPPPPPPP
  PPPPERRDAWTQEPLPLDMNPLGSDASQGPLASSIRMLCMAQYLLRNARGQQGLLRPLGPQTRSQVTLER
  QPVHNPRQEAPIILLQSPAPPRFTPVPMVALGHTLQPTPPPRPTLPQPRIPLIIPPRHTNQPATTPPTAP
  QRLTLGHQLSLPPHPPPHQSTPHCSSDSTGLPPPPTSYSIPSMTLSPEPLPPPAAPAHPLPGVIYDQQAL
  PPTPGPPWWPPVRDPTPTTQTPPTNTKQGPDQGQGRGRWRGRGRSKGRGRMHKLPEPRRPGPDTSSPSMP
  QLSPVVSLHQGQGPENSPTPGPSTAGPVCRVTPSATPDISPIHEPESSDSEEPPFLFPSDWYPPTLEPAE
  LDESWEGIFETTESHSSDEENVGGPSKRPRTSTQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_009334
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_009334.1, YP_001129441
• RefSeq ORF: 1362 bp
• Locus ID: 5176198
• MW: 49.0 kDa