alpha 1 Catenin (CTNNA1) (NM_001290312) Human Untagged Clone

CAT#: SC336673

CTNNA1 (untagged) - Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha 1 Catenin
• Synonyms: CAP102; MDPT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336673 representing NM_001290312.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCAAGAAGACCAGGGACTTGCGTAGACAGCTCCGCAAAGCTGTCATGGACCACGTTTCAGATTCT
  TTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAGCTGCAAAGAATGGAAATGAGAAAGAAGTT
  AAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATTGATTGAGGTTGCCAACTTGGCCTGTTCC
  ATCTCAAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCTGCAAGCCAGTTAGAAGCCCTCTGTCCT
  CAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGAGTAAACTGGCCCAAGAGAACATGGAT
  CTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGATGCTGTCGATGACATTACTTCCATT
  GATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAAGATGTGAACAAATGTGTCATTGCTCTCCAA
  GAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGAGGCCGGGCAGCCCGGGTCATTCAC
  GTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGAGAAGGTTCTGGAAGCCACTAAG
  CTGCTCTCCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCAGCCGTGGAAGCCCTCAGCTCG
  GACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGATGCTTCCCGCCTGGTATATGATGGCATCCGG
  GACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGACTCTGACTTTGAGACAGAA
  GATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTGATAGCTGGCCAGAGTGCC
  CGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAACAGGTGGCCAGCTTCCAG
  GAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAATGACATCATTGTGCTG
  GCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAAGGACCACTCAAAAAT
  ACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGGACAAGCTTGGCCGC
  ACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACCTGCAACGCATCGCC
  CTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTCGGCGGGGAGCTT
  GTTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGAATGCTGTGGTG
  CAGACAGTGAAGGCATCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGCTTCCCTCAAC
  CTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAGAAACAGGAT
  GAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCCTCAGCGAG
  TTCAAAGCTATGGACAGCATCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290312
• Insert Size: 1611 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290312.1
• RefSeq Size: 3121 bp
• RefSeq ORF: 1611 bp
• Locus ID: 1495
• UniProt ID: P35221
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
• MW: 59.6 kDa
• Gene Summary: This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]
  Transcript Variant: This variant (5) contains an alternate exon in place of the first seven exons compared to variant 1. This difference causes translation initiation at a downstream AUG and results in an isoform (5) with a shorter N-terminus compared to isoform 1.