KCNT1 (NM_001272003) Human Untagged Clone

CAT#: SC333183

KCNT1 (untagged) - Homo sapiens potassium channel, subfamily T, member 1 (KCNT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNT1
• Synonyms: bA100C15.2; DEE14; EIEE14; ENFL5; KCa4.1; SLACK; Slo2.2
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC333183 representing NM_001272003.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGCCACTCCCTGACGGGGCGCGGACCCCGGGGGGCGTCTGCCGGGAGGCGCGCGGCGGGGGCTACACC
  AACCGGACCTTCGAGTTTGACGACGGCCAATGCGCCCCCAGGGTCCAGGTGGAGTTCTACGTCAACGAG
  AACACCTTCAAGGAGCGGCTCAAGCTGTTCTTCATCAAAAACCAAAGATCGAGCCTGAGGATCCGGCTG
  TTCAACTTCTCCCTGAAGCTGCTCACCTGCCTGCTCTACATTGTGCGCGTCCTGCTCGATGACCCGGCC
  CTGGGCATCGGATGCTGGGGCTGCCCAAAGCAGAACTACTCCTTCAATGACTCGTCCTCCGAGATCAAC
  TGGGCTCCTATTCTGTGGGTGGAGAGAAAGATGACACTGTGGGCGATCCAGGTCATCGTGGCCATAATA
  AGCTTCCTGGAGACGATGCTTCTCATCTACCTCAGCTACAAAGGCAACATCTGGGAGCAGATCTTCCGC
  GTGTCCTTCGTCCTGGAGATGATCAACACTCTGCCCTTCATCATCACGATCTTCTGGCCGCCGCTGCGG
  AACCTGTTCATCCCCGTCTTTCTGAACTGCTGGCTGGCCAAGCACGCGCTGGAAAACATGATTAATGAC
  TTCCACCGTGCCATCCTGCGGACACAGTCAGCCATGTTCAACCAGGTCCTCATCCTCTTCTGCACCCTG
  CTGTGCCTCGTTTTCACGGGGGGTTGCAGGACCTGCGGCATCCAGCACCTGGAGCGGGCGGGCGAGAAC
  CTGTCCCTCCTGACCTCCTTCTACTTCTGCATCGTCACCTTCTCCACCGTGGGCTACGGTGACGTCACG
  CCCAAGATCTGGCCATCGCAGCTGCTGGTGGTCATCATGATCTGCGTGGCCCTCGTGGTGCTCCCACTG
  CAGTTCGAGGAGCTCGTCTACCTCTGGATGGAGCGGCAGAAGTCAGGGGGCAACTACAGCCGCCACCGT
  GCGCAGACGGAGAAGCACGTGGTCCTGTGTGTCAGCTCCCTCAAGATCGACCTTCTCATGGACTTCCTG
  AACGAGTTCTACGCCCACCCCCGGCTCCAGGACTATTACGTGGTCATCCTGTGCCCCACGGAGATGGAT
  GTCCAGGTGCGCAGAGTCCTGCAGATCCCTCTGTGGTCCCAGCGGGTCATCTACCTCCAGGGCTCTGCA
  CTCAAAGACCAGGACCTCATGCGAGCCAAGATGGACAATGGGGAGGCCTGCTTCATCCTCAGCAGCAGG
  AACGAGGTGGACCGCACGGCTGCAGACCACCAGACCATCCTGCGCGCCTGGGCCGTGAAGGACTTCGCC
  CCCAACTGCCCCCTCTACGTCCAGATCCTCAAACCTGAAAACAAGTTTCACGTCAAGTTTGCTGACCAC
  GTGGTGTGTGAGGAGGAGTGCAAGTACGCCATGCTGGCGCTGAACTGCATCTGCCCGGCGACCTCCACC
  CTCATCACCCTGCTGGTGCACACGTCCCGCGGCCAGGAGGGACAGGAGTCTCCGGAGCAGTGGCAGCGC
  ATGTATGGGCGCTGCTCCGGCAACGAGGTGTACCACATCCGCATGGGTGACAGCAAGTTCTTCCGCGAG
  TACGAGGGCAAGAGCTTCACCTACGCGGCCTTCCACGCCCACAAGAAGTATGGCGTGTGCCTCATCGGG
  CTGAAGCGGGAGGACAACAAGAGCATCCTGCTGAACCCGGGGCCCCGGCACATCCTGGCCGCCTCTGAC
  ACCTGCTTCTACATCAACATCACCAAGGAGGAGAACTCGGCCTTCATCTTCAAGCAGGAGGAGAAGCGG
  AAGAAGAGGGCCTTCTCGGGGCAGGGGCTGCACGAGGGTCCGGCCCGCCTGCCCGTGCACAGCATCATC
  GCCTCCATGGGGACAGTGGCCATGGACCTGCAGGGCACAGAGCACCGGCCTACGCAGAGCGGCGGTGGG
  GGCGGGGGCAGCAAGCTGGCACTGCCCACGGAGAACGGCTCGGGCAGCCGGCGGCCCAGCATCGCGCCC
  GTCCTGGAACTGGCCGACAGCTCAGCCCTGCTGCCCTGCGACCTGCTGAGCGACCAGTCGGAGGATGAG
  GTGACGCCGTCGGACGACGAGGGGCTCTCCGTGGTAGAGTATGTGAAGGGCTACCCTCCCAACTCGCCC
  TACATCGGCAGCTCCCCAACCCTGTGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCGGCTG
  GACAAGGGCTGCAAGCACAACAGCTATGAAGACGCCAAGGCCTACGGGTTCAAGAACAAGCTGATCATC
  GTCTCGGCAGAGACGGCCGGCAATGGGCTGTACAACTTCATCGTGCCACTGCGGGCCTACTACAGATCC
  CGCAAGGAGCTGAACCCCATCGTGCTGCTGCTGGACAACAAGCCCGACCACCACTTCCTGGAAGCCATC
  TGCTGCTTCCCCATGGTCTACTACATGGAGGGCTCTGTGGACAACCTGGACAGCCTGCTGCAGTGTGGC
  ATCATCTATGCGGACAACCTGGTGGTGGTGGACAAGGAGAGCACCATGAGCGCCGAGGAGGACTACATG
  GCGGACGCCAAGACCATCGTCAACGTGCAGACCATGTTCCGGCTCTTCCCCAGCCTCAGCATCACCACG
  GAGCTCACCCACCCTTCCAACATGCGCTTCATGCAGTTCCGCGCCAAGGACAGCTACTCTCTGGCTCTT
  TCCAAACTAGAAAAGAGGGAGCGAGAGAATGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCGTTCGCC
  GCCGGCCGCGTCTTCAGCATCAGCATGTTGGACACACTGCTCTACCAGTCCTTCGTGAAGGACTACATG
  ATCACCATCACCCGGCTGCTGCTGGGCCTGGACACCACGCCGGGCTCGGGGTACCTCTGTGCCATGAAA
  ATCACCGAGGGCGACCTGTGGATCCGCACGTACGGCCGCCTCTTCCAGAAGCTCTGCTCCTCCAGCGCC
  GAGATCCCCATTGGCATCTACCGGACAGAGAGCCACGTCTTCTCCACCTCGGAGCCCCACGACCTCAGA
  GCCCAGTCCCAGATCTCGGTGAACGTGGAGGACTGTGAGGACACACGGGAAGTGAAGGGGCCCTGGGGC
  TCCCGCGCTGGCACCGGAGGCAGCTCCCAGGGCCGCCACACGGGCGGCGGTGACCCCGCAGAGCACCCA
  CTGCTACGGCGCAAGAGCCTGCAGTGGGCCCGGAGGCTGAGCCGCAAGGCGCCCAAGCAGGCAGGCCGG
  GCGGCGGCCGCGGAGTGGATCAGCCAGCAGCGCCTCAGCCTGTACCGGCGCTCTGAGCGCCAGGAGCTC
  TCCGAGCTGGTGAAGAACCGCATGAAGCACCTGGGGCTGCCCACCACCGGCTACGAGGACGTAGCAAAT
  TTAACAGCCAGTGATGTCATGAATCGGGTAAACCTGGGATATTTGCAAGACGAGATGAACGACCACCAG
  AACACCCTCTCCTACGTCCTCATCAACCCTCCGCCCGACACGAGGCTGGAGCCCAGTGACATTGTCTAT
  CTCATCCGCTCCGACCCCCTGGCTCACGTGGCCAGCAGCTCCCAGAGCCGGAAGAGCAGCTGCAGCCAC
  AAGCTGTCGTCCTGCAACCCCGAGACTCGCGACGAGACACAGCTCTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272003
• Insert Size: 3636 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272003.1
• RefSeq Size: 4696 bp
• RefSeq ORF: 3636 bp
• Locus ID: 57582
• UniProt ID: Q5JUK3
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 137 kDa
• Gene Summary: Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a sodium-activated potassium channel subunit which is thought to function in ion conductance and developmental signaling pathways. Mutations in this gene cause the early-onset epileptic disorders, malignant migrating partial seizures of infancy and autosomal dominant nocturnal frontal lobe epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, uses an alternate splice site in the 5' region and contains an additional exon in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.