CD73 (NT5E) (NM_001204813) Human Untagged Clone

CAT#: SC329766

NT5E (untagged) - Homo sapiens 5'-nucleotidase, ecto (CD73) (NT5E), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD73
• Synonyms: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC329766 representing NM_001204813.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGTGTCCCCGAGCCGCGCGGGCGCCCGCGACGCTACTCCTCGCCCTGGGCGCGGTGCTGTGGCCTGCG
  GCTGGCGCCTGGGAGCTTACGATTTTGCACACCAACGACGTGCACAGCCGGCTGGAGCAGACCAGCGAG
  GACTCCAGCAAGTGCGTCAACGCCAGCCGCTGCATGGGTGGCGTGGCTCGGCTCTTCACCAAGGTTCAG
  CAGATCCGCCGCGCCGAACCCAACGTGCTGCTGCTGGACGCCGGCGACCAGTACCAGGGCACTATCTGG
  TTCACCGTGTACAAGGGCGCCGAGGTGGCGCACTTCATGAACGCCCTGCGCTACGATGCCATGGCACTG
  GGAAATCATGAATTTGATAATGGTGTGGAAGGACTGATCGAGCCACTCCTCAAAGAGGCCAAATTTCCA
  ATTCTGAGTGCAAACATTAAAGCAAAGGGGCCACTAGCATCTCAAATATCAGGACTTTATTTGCCATAT
  AAAGTTCTTCCTGTTGGTGATGAAGTTGTGGGAATCGTTGGATACACTTCCAAAGAAACCCCTTTTCTC
  TCAAATCCAGGGACAAATTTAGTGTTTGAAGATGAAATCACTGCATTACAACCTGAAGTAGATAAGTTA
  AAAACTCTAAATGTGAACAAAATTATTGCACTGGGACATTCGGGTTTTGAAATGGATAAACTCATCGCT
  CAGAAAGTGAGGGGTGTGGACGTCGTGGTGGGAGGACACTCCAACACATTTCTTTACACAGGCAATCCA
  CCTTCCAAAGAGGTGCCTGCTGGGAAGTACCCATTCATAGTCACTTCTGATGATGGGCGGAAGGTTCCT
  GTAGTCCAGGCCTATGCTTTTGGCAAATACCTAGGCTATCTGAAGATCGAGTTTGATGAAAGAGGAAAC
  GTCATCTCTTCCCATGGAAATCCCATTCTTCTAAACAGCAGCATTCCTGAAGATCCAAGCATAAAAGCA
  GACATTAACAAATGGAGGATAAAATTGGATAATTATTCTACCCAGGAATTAGGGAAAACAATTGTCTAT
  CTGGATGGCTCCTCTCAATCATGCCGCTTTAGAGAATGCAACATGGGCAACCTGATTTGTGATGCAATG
  ATTAACAACAACCTGAGACACACGGATGAAATGTTCTGGAACCACGTATCCATGTGCATTTTAAATGGA
  GGTGGTATCCGGTCGCCCATTGATGAACGCAACAATGGAATCCATGTGGTGTATGATCTTTCCCGAAAA
  CCTGGAGACAGAGTAGTCAAATTAGATGTTCTTTGCACCAAGTGTCGAGTGCCCAGTTATGACCCTCTC
  AAAATGGACGAGGTATATAAGGTGATCCTCCCAAACTTCCTGGCCAATGGTGGAGATGGGTTCCAGATG
  ATAAAAGATGAATTATTAAGACATGACTCTGGTGACCAAGATATCAACGTGGTTTCTACATATATCTCC
  AAAATGAAAGTAATTTATCCAGCAGTTGAAGGTCGGATCAAGTTTTCCACAGGAAGTCACTGCCATGGA
  AGCTTTTCTTTAATATTTCTTTCACTTTGGGCAGTGATCTTTGTTTTATACCAATAG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001204813
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001204813.1
• RefSeq Size: 3936 bp
• RefSeq ORF: 1575 bp
• Locus ID: 4907
• UniProt ID: P21589
• Cytogenetics: 6q14.3
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• MW: 57.9 kDa
• Gene Summary: The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.