splicing factor 1 (SF1) (NM_001178031) Human Untagged Clone

CAT#: SC328987

SF1 (untagged)-Human splicing factor 1 (SF1) transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SF1
• Synonyms: BBP; D11S636; MBBP; ZCCHC25; ZFM1; ZNF162
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001178031, the custom clone sequence may differ by one or more nucleotides
  ATGGAACAGAAGACAGTGATTCCAGGAATGCCTACAGTTATTCCCCCTGGACTTACTCGA
  GAACAAGAAAGAGCTTATATAGTGCAACTGCAGATAGAAGACCTGACTCGTAAACTGCGC
  ACAGGAGACCTGGGCATCCCCCCTAACCCTGAGGACAGGTCCCCTTCCCCTGAGCCCATC
  TACAATAGCGAGGGGAAGCGGCTTAACACCCGAGAGTTCCGCACCCGCAAAAAGCTGGAA
  GAGGAGCGGCACAACCTCATCACAGAGATGGTTGCACTCAATCCGGATTTCAAGCCACCT
  GCAGATTACAAACCTCCAGCAACACGTGTGAGTGATAAAGTCATGATTCCACAAGATGAG
  TACCCAGAAATCAACTTTGTGGGGCTGCTCATCGGGCCCAGAGGGAACACCCTGAAGAAC
  ATAGAGAAGGAGTGCAATGCCAAGATTATGATCCGGGGGAAAGGGTCTGTGAAAGAAGGG
  AAGGTTGGGCGCAAAGATGGCCAGATGTTGCCAGGAGAAGATGAGCCACTTCATGCCCTG
  GTTACTGCCAATACAATGGAGAACGTCAAAAAGGCAGTGGAACAGATAAGAAACATCCTG
  AAGCAGGGTATCGAGACTCCAGAGGACCAGAATGATCTACGGAAGATGCAGCTTCGGGAG
  TTGGCTCGCTTAAATGGGACCCTTCGGGAAGACGATAACAGGATCTTAAGACCCTGGCAG
  AGCTCAGAGACCCGCAGCATTACCAACACCACAGTGTGTACCAAGTGTGGAGGGGCTGGC
  CACATTGCTTCAGACTGTAAATTCCAAAGGCCTGGTGATCCTCAGTCAGCTCAGGATAAA
  GCACGGATGGATAAAGAATATTTGTCCCTCATGGCTGAACTGGGTGAAGCACCTGTCCCA
  GCATCTGTGGGCTCCACCTCTGGGCCTGCCACCACACCCCTGGCCAGCGCACCTCGTCCT
  GCTGCTCCCGCCAACAACCCACCTCCACCGTCTCTCATGTCTACCACCCAGAGCCGCCCA
  CCCTGGATGAATTCTGGCCCTTCAGAGAGTCGGCCCTACCACGGCATGCATGGAGGTGGT
  CCTGGTGGGCCCGGAGGTGGCCCCCACAGCTTCCCACACCCATTACCCAGCCTGACAGGT
  GGGCATGGTGGACATCCCATGCAGCACAACCCCAATGGACCCCCACCCCCTTGGATGCAG
  CCACCACCACCACCGATGAACCAGGGCCCCCACCCTCCTGGGCACCATGGCCCTCCTCCA
  ATGGATCAGTACCTGGGAAGTACGCCTGTGGGCTCTGGGGTCTATCGCCTGCATCAAGGA
  AAAGGTATGATGCCGCCACCACCTATGGGCATGATGCCGCCGCCGCCGCCGCCTCCCAGT
  GGGCAGCCCCCACCCCCTCCCTCTGGTCCTCTTCCCCCATGGCAACAACAGCAGCAGCAG
  CCTCCGCCACCCCCTCCGCCCAGCAGCAGTATGGCTTCCAGTACCCCCTTGCCATGGCAG
  CAAAATACGACGACTACCACCACGAGCGCTGGCACAGGGTCCATCCCGCCATGGCAACAG
  CAGCAGGCGGCTGCCGCAGCTTCTCCAGGAGCCCCTCAGATGCAAGGCAACCCCACTATG
  GTGCCCCTGCCCCCCGGGGTCCAGCCGCCTCTGCCGCCTGGGGCCCCTCCCCCTCCGCCG
  CCTCCACCGCCTGGTTCCGCCGGCATGATGTATGCCCCGCCCCCTCCTCCTCCGCCTCCC
  ATGGACCCTTCTAACTTTGTCACCATGATGGGCATGGGGGTGGCGGGCATGCCGCCCTTC
  GGGATGCCTCCAGCTCCCCCACCGCCTCCACCACAGAACTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001178031
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178031.1, NP_001171502.1
• RefSeq Size: 3365 bp
• RefSeq ORF: 1842 bp
• Locus ID: 7536
• UniProt ID: Q15637
• Cytogenetics: 11q13.1
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
  Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 5, which has a shorter N-terminus, compared to isoform 1.