GSPT1 (NM_002094) Human Untagged Clone

CAT#: SC327953

GSPT1 (untagged)-Human G1 to S phase transition 1 (GSPT1) transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSPT1
• Synonyms: 551G9.2; eRF3a; ETF3A; GST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC327953 representing NM_002094.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATCCGGGCAGTGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGAGCAGCAGCGGCAGCAGCAGC
  AGCGACTCGGCGCCTGACTGCTGGGACCAGGCGGACATGGAAGCCCCCGGGCCGGGCCCTTGCGGCGGC
  GGCGGCTCCCTGGCGGCGGCGGCCGAGGCCCAGCGGGAGAACCTCAGCGCGGCCTTCAGCCGGCAACTC
  AACGTCAACGCCAAGCCCTTCGTGCCCAACGTCCACGCCGCCGAGTTCGTGCCGTCCTTCCTGCGGGGC
  CCGGCAGCGCCGCCACCCCCAGCTGGCGGCGCCGCCAATAACCACGGAGCCGGCAGCGGCGCGGGAGGC
  CGTGCGGCACCTGTGGAATCCTCTCAAGAGGAACAGTCATTGTGTGAAGGTTCAAATTCAGCTGTTAGC
  ATGGAACTTTCAGAACCTATTGTAGAAAATGGAGAGACAGAAATGTCTCCAGAAGAATCATGGGAGCAC
  AAAGAAGAAATAAGTGAAGCAGAGCCAGGGGGTGGTTCCTTGGGAGATGGAAGGCCGCCAGAGGAAAGT
  GCCCATGAAATGATGGAGGAGGAAGAGGAAATCCCAAAACCTAAGTCTGTGGTTGCACCGCCAGGTGCT
  CCTAAGAAAGAGCATGTAAATGTAGTATTCATTGGGCACGTAGATGCTGGCAAGTCAACCATTGGAGGA
  CAAATAATGTATTTGACTGGAATGGTTGACAAAAGGACGCTTGAAAAGTATGAAAGAGAAGCTAAAGAG
  AAAAACAGAGAAACTTGGTACTTGTCTTGGGCCTTAGACACAAATCAGGAAGAACGAGACAAGGGTAAA
  ACAGTAGAAGTGGGTCGTGCCTATTTTGAAACCGAAAAGAAGCATTTCACAATTCTAGATGCCCCTGGC
  CACAAGAGTTTTGTCCCAAATATGATTGGTGGTGCCTCTCAAGCTGATTTGGCTGTGCTGGTAATCTCA
  GCCAGGAAAGGAGAGTTTGAAACTGGATTTGAAAAAGGAGGACAGACAAGAGAACATGCAATGTTGGCA
  AAGACAGCAGGTGTAAAACACCTAATTGTGCTAATTAATAAGATGGATGATCCAACAGTAAATTGGAGC
  AATGAGAGATATGAAGAATGTAAGGAGAAACTAGTGCCATTTTTGAAAAAAGTTGGCTTCAATCCCAAA
  AAGGACATTCACTTTATGCCCTGCTCAGGACTTACTGGAGCAAATCTCAAAGAGCAGTCGGATTTCTGT
  CCTTGGTACATTGGATTACCGTTTATTCCATATCTGGATAATTTGCCGAACTTCAATAGATCAGTTGAT
  GGACCAATCAGGCTGCCAATTGTGGATAAGTACAAGGATATGGGCACTGTGGTCCTGGGAAAGCTGGAA
  TCAGGATCTATTTGTAAAGGCCAGCAGCTTGTGATGATGCCAAACAAGCACAACGTGGAAGTTCTTGGA
  ATACTTTCCGATGATGTAGAGACTGATACCGTAGCCCCAGGTGAAAACCTCAAAATCAGACTGAAAGGA
  ATTGAAGAAGAGGAGATTCTTCCAGGGTTTATACTTTGTGATCCTAATAATCTTTGTCATTCTGGACGC
  ACATTTGATGCCCAGATAGTGATTATAGAGCACAAATCCATCATCTGCCCAGGCTATAATGCGGTGCTG
  CATATTCATACCTGTATTGAGGAGGTGGAAATAACAGCCTTAATCTGCTTGGTAGACAAAAAATCAGGA
  GAAAAAAGTAAGACCCGACCCCGTTTTGTCAAACAAGATCAAGTATGCATTGCTCGCTTAAGGACAGCA
  GGAACCATCTGCCTTGAGACCTTTAAAGACTTCCCTCAGATGGGTCGTTTCACCTTAAGAGATGAGGGT
  AAGACCATTGCAATTGGAAAAGTTCTGAAACTGGTTCCAGAGAAAGACTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002094
• Insert Size: 1914 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002094.3
• RefSeq Size: 7169 bp
• RefSeq ORF: 1914 bp
• Locus ID: 2935
• Cytogenetics: 16p13.13
• Domains: GTP_EFTU, GTP_EFTU_D3, GTP_EFTU_D2
• Protein Families: Transmembrane
• MW: 68.7 kDa
• Gene Summary: Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth (PubMed:2511002). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:24486019).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.