POLR3B (NM_001160708) Human Untagged Clone

SKU SC327695

POLR3B (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide B (POLR3B) transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: POLR3B
• Synonyms: C128; HLD8; INMAP; RPC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001160708, the custom clone sequence may differ by one or more nucleotides; ATGAAAGCCAATGAAAAGGTTACAAGTGACGCTGACCCTATGTGGTACTTAAAATATCTT; AATATCTATGTTGGGCTTCCTGATGTTGAAGAAAGCTTCAATGTAACTAGACCAGTGTCC; CCTCATGAGTGCCGTTTGAGAGACATGACATACTCTGCCCCTATTACAGTGGATATTGAA; TATACCCGAGGCAGCCAGAGGATCATCCGCAATGCCTTACCTATCGGCAGAATGCCCATA; ATGCTACGTAGTTCAAACTGTGTTCTTACAGGAAAAACGCCAGCAGAATTTGCCAAACTG; AACGAATGTCCCTTAGATCCAGGTGGCTACTTCATTGTTAAAGGAGTAGAAAAAGTTATT; CTTATCCAAGAGCAGCTGTCTAAGAACAGGATCATCGTGGAGGCTGATAGAAAAGGGGCT; GTTGGAGCTTCAGTTACCAGCTCTACCCATGAGAAAAAAAGCAGAACCAATATGGCTGTG; AAACAAGGACGATTTTATTTGAGGCATAATACTTTGTCAGAAGATATACCCATTGTCATC; ATATTTAAGGCCATGGGTGTTGAGAGTGACCAGGAAATTGTGCAGATGATTGGAACAGAG; GAGCACGTGATGGCTGCATTTGGGCCCAGTCTGGAAGAGTGCCAGAAAGCTCAGATTTTC; ACACAGATGCAGGCATTAAAATATATAGGGAACAAAGTAAGAAGGCAAAGGATGTGGGGA; GGTGGACCAAAGAAAACCAAAATAGAAGAAGCAAGAGAGCTCCTGGCTTCCACCATTCTG; ACCCATGTCCCAGTTAAGGAATTCAATTTCCGAGCCAAATGTATCTATACTGCAGTGATG; GTGCGAAGAGTTATTCTGGCCCAAGGAGATAATAAAGTTGACGACAGAGATTATTATGGT; AACAAGCGACTGGAATTGGCAGGACAGCTTTTATCTCTTCTTTTTGAAGACTTGTTCAAA; AAATTTAATTCTGAAATGAAAAAGATTGCCGACCAGGTGATTCCTAAGCAAAGAGCAGCC; CAGTTTGATGTTGTCAAACACATGCGCCAAGACCAGATCACCAATGGCATGGTGAATGCT; ATTTCTACCGGAAATTGGTCTTTAAAGAGATTTAAAATGGACCGCCAGGGTGTAACCCAA; GTGCTGTCTCGCTTGTCATATATATCCGCACTGGGCATGATGACAAGAATCTCTTCCCAG; TTTGAAAAAACGAGAAAAGTGAGTGGTCCTCGCTCCCTCCAGCCATCTCAGTGGGGAATG; CTGTGTCCTTCGGACACTCCTGAAGGAGAGGCATGTGGTTTGGTTAAAAACTTGGCCCTT; ATGACACACATCACAACTGATATGGAAGATGGACCCATTGTTAAATTAGCCAGTAACTTG; GGAGTAGAAGATGTGAATTTATTATGTGGGGAAGAGCTCTCTTACCCAAATGTGTTTCTT; GTCTTTCTTAATGGTAACATCTTAGGTGTCATTCGAGACCACAAAAAGCTAGTGAATACA; TTTCGACTCATGAGAAGAGCAGGATATATCAATGAATTTGTTTCCATCTCAACAAATCTT; ACAGATCGATGTGTCTATATTTCTTCTGATGGGGGAAGGCTATGCAGACCCTACATAATT; GTCAAGAAACAGAAGCCAGCAGTCACAAATAAACATATGGAAGAGCTGGCCCAAGGGTAC; AGGAATTTTGAAGATTTCTTACATGAGAGTCTGGTTGAATATTTAGATGTGAATGAAGAA; AATGATTGTAACATTGCACTGTACGAACACACAATTAATAAAGACACCACCCACTTGGAG; ATTGAACCCTTCACTCTTCTCGGCGTGTGTGCTGGACTTATCCCATACCCTCACCATAAC; CAGTCACCGAGAAACACTTATCAGTGTGCCATGGGGAAACAAGCCATGGGTACTATAGGA; TACAACCAGCGAAACAGAATTGATACTCTCATGTATCTACTAGCATATCCACAAAAACCC; ATGGTTAAGACAAAAACCATTGAATTGATAGAATTTGAGAAACTGCCAGCTGGACAGAAT; GCAACAGTTGCTGTGATGAGCTATAGTGGCTATGATATTGAAGATGCTCTTGTTTTAAAC; AAGGCCTCTTTAGACAGAGGCTTTGGGCGTTGCCTTGTATATAAAAATGCTAAATGTACG; TTGAAACGATACACCAATCAGACTTTTGATAAAGTGATGGGGCCCATGTTGGATGCTGCT; ACAAGGAAACCTATCTGGCGACATGAAATCTTAGATGCAGATGGTATTTGTTCTCCAGGT; GAGAAAGTAGAAAACAAACAAGTGCTTGTAAATAAGTCCATGCCCACAGTGACTCAGATT; CCTTTGGAAGGAAGTAATGTACCACAGCAACCACAGTACAAAGATGTACCCATAACCTAC; AAAGGAGCAACAGACTCATATATTGAAAAAGTGATGATATCTTCAAATGCTGAAGATGCT; TTTCTGATCAAAATGCTGCTGAGACAGACAAGGCGTCCAGAAATTGGAGACAAATTCAGC; AGTCGTCATGGGCAAAAAGGTGTTTGTGGCTTGATCGTCCCCCAGGAAGACATGCCATTT; TGTGATTCTGGCATCTGTCCGGACATCATCATGAACCCACACGGCTTCCCATCACGAATG; ACGGTGGGGAAGCTCATTGAGCTGCTGGCTGGCAAGGCCGGTGTGCTGGACGGCAGATTC; CACTACGGCACTGCGTTTGGAGGCAGTAAAGTGAAGGATGTGTGTGAGGACCTCGTTCGC; CATGGTTATAACTACTTGGGGAAAGACTATGTTACATCCGGCATCACAGGTGAGCCCTTA; GAAGCATACATCTATTTTGGCCCCGTGTACTATCAGAAGCTGAAACACATGGTGCTAGAT; AAAATGCATGCCCGGGCCCGGGGCCCACGAGCCGTCCTTACCAGGCAACCCACTGAAGGA; CGGTCTCGTGATGGTGGCTTGCGTCTCGGGGAAATGGAACGTGACTGTTTAATCGGTTAT; GGAGCCAGTATGCTTTTGCTAGAGAGACTAATGATTTCAAGTGATGCCTTTGAGGTTGAT; GTCTGTGGGCAGTGTGGACTTCTGGGGTATTCTGGCTGGTGCCATTACTGCAAGTCATCC; TGCCACGTGTCTTCCCTCCGTATTCCGTATGCCTGCAAGCTGCTCTTCCAGGAACTACAG; TCTATGAACATCATCCCCAGGTTAAAACTGTCCAAGTACAATGAA;
• Restriction Sites: Please inquire
• ACCN: NM_001160708
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001160708.1, NP_001154180.1
• RefSeq Size: 4133 bp
• RefSeq ORF: 3228 bp
• Locus ID: 55703
• UniProt ID: Q9NW08
• Cytogenetics: 12q23.3
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Summary: This gene encodes the second largest subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The largest subunit and the encoded protein form the catalytic center of RNA polymerase III. Mutations in this gene are a cause of hypomyelinating leukodystrophy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]; Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an in-frame downstream AUG, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.