ROBO1 (NM_001145845) Human Untagged Clone

CAT#: SC326514

ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 4, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO1
• Synonyms: DUTT1; SAX3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145845, the custom clone sequence may differ by one or more nucleotides
  ATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCC
  CGTCTTCGTCAGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTC
  TCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATT
  GAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGA
  ATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGA
  CCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCAC
  AATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTC
  ATGGTTGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAG
  CCCACCATTTCATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACT
  ATACGAGGAGGAAAGCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTT
  TGTGTTGGTACCAATATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTA
  GAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCA
  GAATTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGAT
  GGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAG
  GTGACAGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCT
  GAAGCATCTGCTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAA
  CCCCGTGACCAGGTTGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGA
  AATCCTCAACCAGCTATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATAT
  CAACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACT
  AATGTCCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGC
  ATCATCACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTT
  ATTCGACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGT
  GTGGCCACAGGCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCA
  ACCCAAGACTCTCGAATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAG
  CTGGGTGATACTGGTCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGG
  AGTGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGAC
  CCAAATTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACA
  GTCACATTATCGTGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATA
  GAAGCCTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACA
  GAAACATCTGCCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCA
  GCTAATGCATATGGAATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGAT
  GTCCTACCAACAAGTCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCT
  GTTCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACA
  GTAGATCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCC
  AACCACGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTG
  GTAATCCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAAT
  GAATTTCAAGGAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGT
  GCCCCACCCCAAGGTGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTT
  AGTTGGCAGCCACCTCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGG
  TGTCTGGGCAATGAAACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCC
  GTGGTCATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACT
  GGGGCTGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAAC
  CCTGTGTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAG
  CCGGCCTTCATAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATC
  TGGCTTTATCGACACCGCAAGAAGAGAAACGGACTTACTAGTACCTACGCGGGTATCAGA
  AAAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTC
  AACATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAAC
  AACCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAAC
  CTCACTACCTACAGTCGCCCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAG
  TCAAACCTCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAA
  CCACTGGGACAGCAGAAACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAAC
  AAGCTGAACAAAGATTATCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAA
  TCATACGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCT
  GGGAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATG
  AACTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAA
  GAGTACAACATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCA
  GCAAGGATGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACT
  CCCCCTGTTCGGGGAGCAGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACT
  GCCACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAG
  GAGACTGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCA
  CCACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCA
  GATATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAG
  ATGCAAACCAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGG
  GACCTGGAGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAG
  GACAACATTTCCAGCGGACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGAT
  GCTGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGA
  CGGCAAATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCC
  ACAAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCA
  GCCAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTT
  CCACCACCTCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAG
  CTGGAAGTACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGA
  TCATCAGACAGAAAAGGAAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTT
  GTTGACATGCGAACAAATCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGG
  AAAGGACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATC
  CAAGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGAT
  CCCAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCA
  AATGTAGGTCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAA
  GATAATAATGAAGAATTAGAGGAAACTGAAAGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001145845
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145845.1, NP_001139317.1
• RefSeq Size: 7385 bp
• RefSeq ORF: 4656 bp
• Locus ID: 6091
• UniProt ID: Q9Y6N7
• Cytogenetics: 3p12.3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (4) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, lacks an alternate in-frame exon in the central coding region, and uses an alternate splice site in the central coding region, compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus and differs at three internal regions, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.